ArticleMolecular biology of the cell1997
Differential localization of myosin and myosin phosphatase subunits in smooth muscle cells and migrating fibroblasts.
Article in Molecular biology of the cell, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 61 citations in OpenAlex.
- Rediscovery of PHI-1/PPP1R14B: Emerging Roles of Cellular PP1 Signaling Mediated by theBiomolecules · 2025Review
- An Interaction Network of the Human SEPT9 Established by Quantitative Mass Spectrometry.G3 (Bethesda, Md.) · 2019Article
- Ankyrin domain of myosin 16 influences motor function and decreases protein phosphatase catalytic activity.European biophysics journal : EBJ · 2015Article
- Arf guanine nucleotide-exchange factors BIG1 and BIG2 regulate nonmuscle myosin IIA activity by anchoring myosin phosphatase complex.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Nuclear localization of CPI-17, a protein phosphatase-1 inhibitor protein, affects histone H3 phosphorylation and corresponds to proliferation of cancer and smooth muscle cells.Biochemical and biophysical research communications · 2013Article
- Mapping the cytoskeletal prestress.American journal of physiology. Cell physiology · 2010Article
- Myosin phosphatase target subunit: Many roles in cell function.Biochemical and biophysical research communications · 2008Review
- A novel regulatory mechanism of myosin light chain phosphorylation via binding of 14-3-3 to myosin phosphatase.Molecular biology of the cell · 2008Article
- Targeting of FAK Ser910 by ERK5 and PP1delta in non-stimulated and phorbol ester-stimulated cells.The Biochemical journal · 2007Article
- Chromosome instability, chromosome transcriptome, and clonal evolution of tumor cell populations.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Novel ZIP kinase isoform lacks leucine zipper.Archives of biochemistry and biophysics · 2006Article
- Regulation of FAK Ser-722 phosphorylation and kinase activity by GSK3 and PP1 during cell spreading and migration.The Biochemical journal · 2005Article
- Localization of myosin phosphatase target subunit and its mutants.Journal of muscle research and cell motility · 2005Article
- Phosphoprotein inhibitor CPI-17 specificity depends on allosteric regulation of protein phosphatase-1 by regulatory subunits.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Identification of genes involved in cell invasion by using a library of randomized hybrid ribozymes.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Phosphorylation of the myosin phosphatase inhibitors, CPI-17 and PHI-1, by integrin-linked kinase.The Biochemical journal · 2002Article
- Phosphorylation of the myosin phosphatase target subunit by integrin-linked kinase.The Biochemical journal · 2002Article
- Cell-cycle-dependent association of protein phosphatase 1 and focal adhesion kinase.The Biochemical journal · 2001Article
- Rho-kinase--mediated contraction of isolated stress fibers.The Journal of cell biology · 2001Article
- Serine phosphorylation of focal adhesion kinase in interphase and mitosis: a possible role in modulating binding to p130(Cas).Molecular biology of the cell · 2001Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Myosin II light chains (MLC20) are phosphorylated by a Ca2+/calmodulin-activated kinase and dephosphorylated by a phosphatase that has been purified as a trimer containing the delta isoform of type 1 catalytic subunit (PP1C delta), a myosin-binding 130-kDa subunit (M130) and a 20-kDa subunit. The distribution of M130 and PP1C as well as myosin II was examined in smooth muscle cells and fibroblasts by immunofluorescence microscopy and immunoblotting after differential extraction. Myosin and M130 colocalized with actin stress fibers in permeabilized cells. However, in nonpermeabilized cells the staining for myosin and M130 was different, with myosin mostly at the periphery of the cell and the M130 appearing diffusely throughout the cytoplasm. Accordingly, most M130 was recovered in a soluble fraction during permeabilization of cells, but the conditions used affected the solubility of both M130 and myosin. The PP1C alpha isoform colocalized with M130 and also was in the nucleus, whereas the PP1C delta isoform was localized prominently in the nucleus and in focal adhesions. In migrating cells, M130 concentrated in the tailing edge and was depleted from the leading half of the cell, where double staining showed myosin II was present. Because the tailing edge of migrating cells is known to contain phosphorylated myosin, inhibition of myosin LC20 phosphatase, probably by phosphorylation of the M130 subunit, may be required for cell migration.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.