ArticlePlant molecular biology1997
Expression of a novel-type small proline-rich protein gene of alfalfa is induced by 2,4-dichlorophenoxiacetic acid in dedifferentiated callus cells.
Article in Plant molecular biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
3 citing papers in PubMed, 28 citations in OpenAlex.
- HyPRP1 Gene Suppressed by Multiple Stresses Plays a Negative Role in Abiotic Stress Tolerance in Tomato.Frontiers in plant science · 2016Article
- A survey of genes differentially expressed during long-term heat-induced chilling tolerance in citrus fruit.Planta · 2003Article
- Partial characterization of genes whose transcripts accumulate preferentially in cell clusters at the earliest stage of carrot somatic embryogenesis.Plant molecular biology · 2001Article
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Authors and funding
7 authors at 5 institutions in 3 countries.
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No grant is acknowledged in the PubMed record.
Abstract
Differential screening of a cDNA library of 2,4-dichlorophenoxiacetic acid (2,4-D)-treated alfalfa (Medicago sativa) callus tissues resulted in the isolation of a 571 bp cDNA clone (MsPRP5) encoding for a proline-rich protein (84 amino acids) with a specific repeat unit of TPVLPPRK/RGRPPPVPP. In addition, a characteristic amino acid block (PPVYK) previously found in other proline-rich proteins also occurs in the C-terminal region of MsPRP5. At the N-terminal, a signal peptide similar to leader sequences of extracellular proteins can be predicted. According to the northern analysis, the corresponding gene is not expressed or is weakly expressed in differentiated vegetative organs and somatic embryos. However the accumulation of MsPRP5 mRNA is auxin concentration-dependent in dedifferentiated callus tissue. An increase in the amount of steady-state mRNA was detected already 20 min after auxin shock (100 microM 2,4-D). Maximum expression was observed at 24-48 h in the presence of 2,4-D. Elevated expression was also found in cells recovering after heat shock and wounding stress. In synchronized alfalfa cells, the transcript level of MsPRP5 gene fluctuated during cell cycle progression with peaks in G1/S phase cells. Considering the structural features and expression properties of MsPRP5, this clone may represents a new type of proline-rich protein gene which responds to hormonal shock and some other stresses as well.
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