ArticleThe Journal of cell biology1997
ADP ribosylation factor 1 is required for synaptic vesicle budding in PC12 cells.
Article in The Journal of cell biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 98 citations in OpenAlex.
- A neurodevelopmental disorder associated with an activating de novo missense variant in ARF1.Human molecular genetics · 2023Article
- SNX5 targets a monoamine transporter to the TGN for assembly into dense core vesicles by AP-3.The Journal of cell biology · 2022Article
- Synaptic Vesicle Recycling Pathway Determines Neurotransmitter Content and Release Properties.Neuron · 2019Article
- CaThe Journal of biological chemistry · 2017Article
- Molecular Machines Determining the Fate of Endocytosed Synaptic Vesicles in Nerve Terminals.Frontiers in synaptic neuroscience · 2016Review
- Synaptic vesicle recycling: steps and principles.The EMBO journal · 2014Review
- Chemical-genetic disruption of clathrin function spares adaptor complex 3-dependent endosome vesicle biogenesis.Molecular biology of the cell · 2013Article
- Multiple dileucine-like motifs direct VGLUT1 trafficking.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- Role of AP1 and Gadkin in the traffic of secretory endo-lysosomes.Molecular biology of the cell · 2011Article
- Clathrin-dependent mechanisms modulate the subcellular distribution of class C Vps/HOPS tether subunits in polarized and nonpolarized cells.Molecular biology of the cell · 2011Article
- Article
- An AP-3-dependent mechanism drives synaptic-like microvesicle biogenesis in pancreatic islet beta-cells.American journal of physiology. Endocrinology and metabolism · 2010Article
- CB(1) cannabinoid receptors and their associated proteins.Current medicinal chemistry · 2010Review
- SLC30A3 (ZnT3) oligomerization by dityrosine bonds regulates its subcellular localization and metal transport capacity.PloS one · 2009Article
- Loss of AP-3 function affects spontaneous and evoked release at hippocampal mossy fiber synapses.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- BLOC-1 complex deficiency alters the targeting of adaptor protein complex-3 cargoes.Molecular biology of the cell · 2006Article
- The Arf-GTPase-activating protein Gcs1p is essential for sporulation and regulates the phospholipase D Spo14p.Eukaryotic cell · 2006Article
- p120-Catenin regulates clathrin-dependent endocytosis of VE-cadherin.Molecular biology of the cell · 2005Article
- Saccharomyces cerevisiae Npc2p is a functionally conserved homologue of the human Niemann-Pick disease type C 2 protein, hNPC2.Eukaryotic cell · 2005Article
- Phosphatidylinositol-4-kinase type II alpha is a component of adaptor protein-3-derived vesicles.Molecular biology of the cell · 2005Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Carrier vesicle generation from donor membranes typically progresses through a GTP-dependent recruitment of coats to membranes. Here we explore the role of ADP ribosylation factor (ARF) 1, one of the GTP-binding proteins that recruit coats, in the production of neuroendocrine synaptic vesicles (SVs) from PC12 cell membranes. Brefeldin A (BFA) strongly and reversibly inhibited SV formation in vivo in three different PC12 cell lines expressing vesicle-associated membrane protein-T Antigen derivatives. Other membrane traffic events remained unaffected by the drug, and the BFA effects were not mimicked by drugs known to interfere with formation of other classes of vesicles. The involvement of ARF proteins in the budding of SVs was addressed in a cell-free reconstitution system (Desnos, C., L. Clift-O'Grady, and R.B. Kelly. 1995. J. Cell Biol. 130:1041-1049). A peptide spanning the effector domain of human ARF1 (2-17) and recombinant ARF1 mutated in its GTPase activity, both inhibited the formation of SVs of the correct size. During in vitro incubation in the presence of the mutant ARFs, the labeled precursor membranes acquired different densities, suggesting that the two ARF mutations block at different biosynthetic steps. Cell-free SV formation in the presence of a high molecular weight, ARF-depleted fraction from brain cytosol was significantly enhanced by the addition of recombinant myristoylated native ARF1. Thus, the generation of SVs from PC12 cell membranes requires ARF and uses its GTPase activity, probably to regulate coating phenomena.
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