ArticleAnalytical biochemistry1997
Sodium Green as a potential probe for intracellular sodium imaging based on fluorescence lifetime.
Article in Analytical biochemistry, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 67 citations in OpenAlex.
- Heart rate changes and myocardial sodium.Physiological reports · 2022Article
- Sodium-Sensitive Contact Lens for Diagnostics of Ocular Pathologies.Sensors and actuators. B, Chemical · 2021Article
- Advances in the development of novel compounds targeting cation-chloride cotransporter physiology.American journal of physiology. Cell physiology · 2021Review
- Fluorescent contact lens for continuous non-invasive measurements of sodium and chloride ion concentrations in tears.Analytical biochemistry · 2020Article
- Quantitative determination of cellular [NaThe Journal of general physiology · 2019Article
- NaChemistry (Weinheim an der Bergstrasse, Germany) · 2019Article
- Live-Cell Imaging of Physiologically Relevant Metal Ions Using Genetically Encoded FRET-Based Probes.Cells · 2019Review
- Contact lens to measure individual ion concentrations in tears and applications to dry eye disease.Analytical biochemistry · 2018Article
- Arabidopsis Qc-SNARE gene AtSFT12 is involved in salt and osmotic stress responses and Na(+) accumulation in vacuoles.Plant cell reports · 2015Article
- Handheld Device Adapted to Smartphone Cameras for the Measurement of Sodium Ion Concentrations at Saliva-Relevant Levels via Fluorescence.Bioengineering (Basel, Switzerland) · 2015Article
- Phasor imaging with a widefield photon-counting detector.Journal of biomedical optics · 2012Article
- Heterologous expression of vacuolar H(+)-PPase enhances the electrochemical gradient across the vacuolar membrane and improves tobacco cell salt tolerance.Protoplasma · 2007Article
- Induction of salt and osmotic stress tolerance by overexpression of an intracellular vesicle trafficking protein AtRab7 (AtRabG3e).Plant physiology · 2004Article
- Potassium and sodium measurements at clinical concentrations using phase-modulation fluorometry.Sensors and actuators. B, Chemical · 1999Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
We characterized the use of the fluorescent probe Sodium Green for measurements of intracellular free sodium using frequency-domain, phase-modulation fluorometry. The intensity decays were found to be strongly Na+ dependent, with mean lifetime increasing from 1.13 ns in the absence of Na+ to 2.39 ns in the presence of 140 mM Na+. Detailed analysis of the intensity decays in the presence of Na+ and K+ in the concentration range from 0 to 500 mM is provided. Sodium sensing using data measured at a single modulation frequency is described. Phase and modulation data showed high sensitivity to Na+ and substantially lower sensitivity to K+. Additionally, exposure of Sodium Green to intense illumination indicated that Sodium Green is much more photostable than its precursor, fluorescein. These results indicate that lifetime-based measurements with Sodium Green can be used for imaging of intracellular free [Na+] in the range from about 0.5 to 50 mM with high accuracy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.