ArticleJournal of bacteriology1997
A new Thermus-Escherichia coli shuttle integration vector system.
Article in Journal of bacteriology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 51 citations in OpenAlex.
- Complete genome sequences ofMicrobiology resource announcements · 2026Article
- Development of newApplied and environmental microbiology · 2025Article
- Complete genome sequences ofMicrobiology resource announcements · 2024Article
- Complete Genome Sequences of Thermus thermophilus Strains HB5002 and HB5008, Isolated from Mine Hot Spring in Japan.Microbiology resource announcements · 2021Article
- Mechanical inhibition of isolated VeLife · 2020Article
- Markerless Gene Deletion with Cytosine Deaminase in Thermus thermophilus Strain HB27.Applied and environmental microbiology · 2016Article
- Engineering the genome of Thermus thermophilus using a counterselectable marker.Journal of bacteriology · 2015Article
- The ingenious structure of central rotor apparatus in VoV1; key for both complex disassembly and energy coupling between V1 and Vo.PloS one · 2015Article
- Molecular basis of ADP inhibition of vacuolar (V)-type ATPase/synthase.The Journal of biological chemistry · 2014Article
- Reconstitution of vacuolar-type rotary H+-ATPase/synthase from Thermus thermophilus.The Journal of biological chemistry · 2012Article
- Genetic tool development underpins recent advances in thermophilic whole-cell biocatalysts.Microbial biotechnology · 2011Review
- Genomic and proteomic characterization of the large Myoviridae bacteriophage ϕTMA of the extreme thermophile Thermus thermophilus.Bacteriophage · 2011Article
- Thermus thermophilus as biological model.Extremophiles : life under extreme conditions · 2009Review
- ATP hydrolysis and synthesis of a rotary motor V-ATPase from Thermus thermophilus.The Journal of biological chemistry · 2008Article
- Characterization of three putative Lon proteases of Thermus thermophilus HB27 and use of their defective mutants as hosts for production of heterologous proteins.Extremophiles : life under extreme conditions · 2008Article
- Structure of a central stalk subunit F of prokaryotic V-type ATPase/synthase from Thermus thermophilus.The EMBO journal · 2005Article
- Membrane-associated maturation of the heterotetrameric nitrate reductase of Thermus thermophilus.Journal of bacteriology · 2005Article
- High-level overproduction of His-tagged Tth DNA polymerase in Thermus thermophilus.Applied and environmental microbiology · 2005Article
- Crystal structure of a central stalk subunit C and reversible association/dissociation of vacuole-type ATPase.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Production of recombinant alpha-galactosidases in Thermus thermophilus.Applied and environmental microbiology · 2001Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We established a Thermus thermophilus strain in which the pyrE gene (coding for orotate phosphoribosyltransferase of the pyrimidine biosynthetic pathway) was totally deleted. We also constructed an integration vector, which consisted of the Escherichia coli plasmid vector pBluescript and a 2.1-kb segment of the T. thermophilus leu operon sequence, for the integration of a foreign gene into a chromosome of the thermophile. pyrE and leuB genes were used as probes to test the integration vector. The integration vector pINV, bearing the pyrE gene, transformed the delta pyrE strain at a frequency of 6 x 10(-5) through a single crossover event. The leuB gene could also be used as another marker of the integration vector system. The vector could be integrated at the expected site. By digesting the chromosomal DNA of the T. thermophilus transformants with a unique restriction enzyme, the vector could be recovered into E. coli after the recircularization in vitro. The kanamycin nucleotidyltransferase gene could be successfully expressed in the thermophile by using pINV.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.