ArticleNucleic acids research1997
RNA-protein interactions within the 3 ' untranslated region of vimentin mRNA.
Article in Nucleic acids research, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 37 citations in OpenAlex.
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- MicroRNA-17-3p is a prostate tumor suppressor in vitro and in vivo, and is decreased in high grade prostate tumors analyzed by laser capture microdissection.Clinical & experimental metastasis · 2009Article
- Hairpin structure within the 3'UTR of DNA polymerase beta mRNA acts as a post-transcriptional regulatory element and interacts with Hax-1.Nucleic acids research · 2007Article
- Review
- Elongation factor 1alpha binds to the region of the metallothionein-1 mRNA implicated in perinuclear localization--importance of an internal stem-loop.RNA (New York, N.Y.) · 2006Article
- An AU-rich stem-loop structure is a critical feature of the perinuclear localization signal of c-myc mRNA.The Biochemical journal · 2005Article
- Perinuclear localization of slow troponin C m RNA in muscle cells is controlled by a cis-element located at its 3' untranslated region.RNA (New York, N.Y.) · 2005Article
- Cell and molecular regulation of endothelin-1 production during hepatic wound healing.Molecular biology of the cell · 2003Article
- Discovery of RNA structural elements using evolutionary computation.Nucleic acids research · 2002Article
- The 3' untranslated region of human vimentin mRNA interacts with protein complexes containing eEF-1gamma and HAX-1.Nucleic acids research · 2002Article
- RNA-protein interactions of the 3' untranslated regions of myosin heavy chain transcripts.Journal of muscle research and cell motility · 2002Article
- UTRdb and UTRsite: specialized databases of sequences and functional elements of 5' and 3' untranslated regions of eukaryotic mRNAs. Update 2002.Nucleic acids research · 2002Article
- RNAMotif, an RNA secondary structure definition and search algorithm.Nucleic acids research · 2001Article
- UTRdb and UTRsite: specialized databases of sequences and functional elements of 5' and 3' untranslated regions of eukaryotic mRNAs.Nucleic acids research · 2000Article
- HIT family genes: FHIT but not PKCI-1/HINT produces altered transcripts in colorectal cancer.British journal of cancer · 1999Article
- Highly conserved RNA sequences that are sensors of environmental stress.Molecular and cellular biology · 1998Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Several functions have been attributed to protein binding within the 3'untranslated region (3'UTR) of mRNA, including mRNA localization, stability, and translational repression. Vimentin is an intermediate filament protein whose 3'untranslated sequence is highly conserved between species. In order to identify sequences that might play a role in vimentin mRNA function, we synthesized32P-labeled RNA from different regions of vimentin's 3'UTR and assayed for protein binding with HeLa extracts using band shift assays. Sequences required for binding are contained within a region 61-114 nucleotides downstream of the stop codon, a region which is highly conserved from Xenopus to man. As judged by competition assays, binding is specific. Solution probing studies of 32P-labeled RNA with various nucleases and lead support a complex stem and loop structure for this region. Finally, UV cross-linking of the RNA-protein complex identifies an RNA binding protein of 46 kDa. Fractionation of a HeLa extract on a sizing column suggests that in addition to the 46 kDa protein, larger complexes containing additional protein(s) can be identified. Vimentin mRNA has been shown to be localized to the perinuclear region of the cytoplasm, possibly at sites of intermediate filament assembly. To date, all sequences required for localization of various mRNAs have been confined to the 3'UTR. Therefore, we hypothesize that this region and associated protein(s) might be important for vimentin mRNA function such as in localization.
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