ArticleProceedings of the National Academy of Sciences of the United States of America1997
Transcription initiation is controlled by three core promoter elements in the hgl5 gene of the protozoan parasite Entamoeba histolytica.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 60 citations in OpenAlex.
- Promoter-Bound Full-Length Intronic Circular RNAs-RNA Polymerase II Complexes Regulate Gene Expression in the Human ParasiteNon-coding RNA · 2022Article
- Entamoeba stage conversion: progress and new insights.Current opinion in microbiology · 2020Review
- Genomic determinants for initiation and length of natural antisense transcripts in Entamoeba histolytica.Scientific reports · 2020Article
- Article
- Transcriptomic analysis reveals novel downstream regulatory motifs and highly transcribed virulence factor genes of Entamoeba histolytica.BMC genomics · 2019Article
- Identification of the gene encoding the TATA box-binding protein-associated factor 1 (TAF1) and its putative role in the heat shock response in the protozoan parasite Entamoeba histolytica.Parasitology research · 2019Article
- The Entamoeba histolytica TBP and TRF1 transcription factors are GAAC-box binding proteins, which display differential gene expression under different stress stimuli and during the interaction with mammalian cells.Parasites & vectors · 2018Article
- Regulation of gene expression in the protozoan parasite Entamoeba invadens: identification of core promoter elements and promoters with stage-specific expression patterns.International journal for parasitology · 2014Article
- Transferrin regulates mRNA levels of a gene involved in iron utilization in Entamoeba histolytica.Molecular biology reports · 2012Article
- Regulation of gene expression in protozoa parasites.Journal of biomedicine & biotechnology · 2010Review
- Recent insights into Entamoeba development: identification of transcriptional networks associated with stage conversion.International journal for parasitology · 2009Review
- Trichostatin A effects on gene expression in the protozoan parasite Entamoeba histolytica.BMC genomics · 2007Article
- Identification of putative transcriptional regulatory networks in Entamoeba histolytica using Bayesian inference.Nucleic acids research · 2007Article
- Initiator recognition in a primitive eukaryote: IBP39, an initiator-binding protein from Trichomonas vaginalis.Molecular and cellular biology · 2001Article
- Molecular epidemiology of Entamoeba spp.: evidence of a bottleneck (Demographic sweep) and transcontinental spread of diploid parasites.Journal of clinical microbiology · 2000Article
- Analysis of a ubiquitous promoter element in a primitive eukaryote: early evolution of the initiator element.Molecular and cellular biology · 1999Article
- Regulation of adherence and virulence by the Entamoeba histolytica lectin cytoplasmic domain, which contains a beta2 integrin motif.Molecular biology of the cell · 1998Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Entamoeba histolytica is a single cell eukaryote that is the etiologic agent of amoebic colitis. Core promoter elements of E. histolytica protein encoding genes include a TATA-like sequence (GTATTTAAAG/C) at -30, a novel element designated GAAC (GAACT) that has a variable location between TATA and the site of transcription initiation, and a putative initiator (Inr) element (AAAAATTCA) overlying the site of transcription initiation. The presence of three separate conserved sequences in a eukaryotic core promoter is unprecedented and prompted examination of their roles in regulating transcription initiation. Alterations of all three regions in the hgl5 gene decreased reporter gene activity with the greatest effect seen by mutation of the GAAC element. Positional analysis of the TATA box demonstrated that transcription initiated consistently 30-31 bases downstream of the TATA region. Mutation of either the TATA or GAAC elements resulted in the appearance of new transcription start sites upstream of +1 in the promoter of the hgl5 gene. Mutation of the Inr element resulted in no change in the site of transcription initiation; however, in the presence of a mutated TATA and GAAC regions, the Inr element controlled the site of transcription initiation. We conclude that all three elements play a role in determining the site of transcription initiation. The variable position of the GAAC element relative to the site of transcription initiation, and the multiple transcription initiations that resulted from its mutation, indicate that the GAAC element has an important and apparently novel role in transcriptional control in E. histolytica.
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