ArticleProceedings of the National Academy of Sciences of the United States of America1997
ECA1 complements yeast mutants defective in Ca2+ pumps and encodes an endoplasmic reticulum-type Ca2+-ATPase in Arabidopsis thaliana.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
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Who cites it
58 citing papers in PubMed, 151 citations in OpenAlex.
- An intracellular CPK-ECA1 phosphoregulatory circuit couples calcium signatures to ABA homeostasis for plant osmosensivity.Science advances · 2025Article
- Inter-Organellar CaCells · 2025Review
- Yeast Heterologous Expression Systems for the Study of Plant Membrane Proteins.International journal of molecular sciences · 2023Review
- The trans-Golgi-localized protein BICAT3 regulates manganese allocation and matrix polysaccharide biosynthesis.Plant physiology · 2022Article
- Structure, Function, and Applications of Soybean Calcium Transporters.International journal of molecular sciences · 2022Review
- Transport, functions, and interaction of calcium and manganese in plant organellar compartments.Plant physiology · 2021Review
- Identification and Expression Profiling Analysis of the Cation/CaFrontiers in genetics · 2021Article
- Manganese in Plants: From Acquisition to Subcellular Allocation.Frontiers in plant science · 2020Review
- NADPH oxidase activity is required for ER stress survival in plants.The Plant journal : for cell and molecular biology · 2018Article
- MIZ1 regulates ECA1 to generate a slow, long-distance phloem-transmitted CaProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Endoplasmic reticulum-localized CCX2 is required for osmotolerance by regulating ER and cytosolic CaProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Antarctic Moss Multiprotein Bridging Factor 1c Overexpression in Arabidopsis Resulted in Enhanced Tolerance to Salt Stress.Frontiers in plant science · 2017Article
- In silico analysis of Mn transporters (NRAMP1) in various plant species.Molecular biology reports · 2016Article
- Mn-euvering manganese: the role of transporter gene family members in manganese uptake and mobilization in plants.Frontiers in plant science · 2014Review
- Analyses of Ca2+ accumulation and dynamics in the endoplasmic reticulum of Arabidopsis root cells using a genetically encoded Cameleon sensor.Plant physiology · 2013Article
- CTR1 phosphorylates the central regulator EIN2 to control ethylene hormone signaling from the ER membrane to the nucleus in Arabidopsis.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- The pmr gene, encoding a Ca2+-ATPase, is required for calcium and manganese homeostasis and normal development of hyphae and conidia in Neurospora crassa.Eukaryotic cell · 2012Article
- Purification, crystallization and preliminary crystallographic studies of a PacL homologue from Listeria monocytogenes.Acta crystallographica. Section F, Structural biology and crystallization communications · 2012Article
- Function and evolution of a MicroRNA that regulates a Ca2+-ATPase and triggers the formation of phased small interfering RNAs in tomato reproductive growth.The Plant cell · 2011Article
- Calcium efflux systems in stress signaling and adaptation in plants.Frontiers in plant science · 2011Article
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Authors and funding
4 authors at 2 institutions in 1 country.
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Abstract
To understand the structure, role, and regulation of individual Ca2+ pumps in plants, we have used yeast as a heterologous expression system to test the function of a gene from Arabidopsis thaliana (ECA1). ECA1 encoded a 116-kDa polypeptide that has all the conserved domains common to P-type Ca2+ pumps (EC 3.6.1.38). The amino acid sequence shared more identity with sarcoplasmic/endoplasmic reticulum (53%) than with plasma membrane (32%) Ca2+ pumps. Yeast mutants defective in a Golgi Ca2+ pump (pmr1) or both Golgi and vacuolar Ca2+ pumps (pmr1 pmc1 cnb1) were sensitive to growth on medium containing 10 mM EGTA or 3 mM Mn2+. Expression of ECA1 restored growth of either mutant on EGTA. Membranes were isolated from the pmr1 pmc1 cnb1 mutant transformed with ECA1 to determine if the ECA1 polypeptide (ECA1p) could be phosphorylated as intermediates of the reaction cycle of Ca2+-pumping ATPases. In the presence of [gamma-32P]ATP, ECA1p formed a Ca2+-dependent [32P]phosphoprotein of 106 kDa that was sensitive to hydroxylamine. Cyclopiazonic acid, a blocker of animal sarcoplasmic/endoplasmic reticulum Ca2+ pumps, inhibited the formation of the phosphoprotein, whereas thapsigargin did not. Immunoblotting with an antibody against the carboxyl tail showed that ECA1p was associated mainly with the endoplasmic reticulum membranes isolated from Arabidopsis plants. The results support the model that ECA1 encodes an endoplasmic reticulum-type Ca2+ pump in Arabidopsis. The ability of ECA1p to restore growth of mutant pmr1 on medium containing Mn2+, and the formation of a Mn2+-dependent phosphoprotein suggested that ECA1p may also regulate Mn2+ homeostasis by pumping Mn2+ into endomembrane compartments of plants.
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