ArticleProceedings of the National Academy of Sciences of the United States of America1997
The U5 RNA of trypanosomes deviates from the canonical U5 RNA: the Leptomonas collosoma U5 RNA and its coding gene.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 47 citations in OpenAlex.
- Structural basis of step II spliced leader RNA trans-splicing in trypanosomatid parasites.Nature communications · 2026Article
- A trans-spliced telomerase RNA dictates telomere synthesis in Trypanosoma brucei.Cell research · 2013Article
- A comparative genome-wide study of ncRNAs in trypanosomatids.BMC genomics · 2010Article
- The pre-mRNA splicing machinery of trypanosomes: complex or simplified?Eukaryotic cell · 2010Review
- Establishment of an in vitro trans-splicing system in Trypanosoma brucei that requires endogenous spliced leader RNA.Nucleic acids research · 2010Article
- 'RNA walk' a novel approach to study RNA-RNA interactions between a small RNA and its target.Nucleic acids research · 2010Article
- Spliceosomal proteomics in Trypanosoma brucei reveal new RNA splicing factors.Eukaryotic cell · 2009Article
- Trypanosome spliced-leader-associated RNA (SLA1) localization and implications for spliced-leader RNA biogenesis.Eukaryotic cell · 2009Article
- Evolution of small nuclear RNAs in S. cerevisiae, C. albicans, and other hemiascomycetous yeasts.RNA (New York, N.Y.) · 2007Article
- Identification of novel snRNA-specific Sm proteins that bind selectively to U2 and U4 snRNAs in Trypanosoma brucei.RNA (New York, N.Y.) · 2007Article
- Sm core variation in spliceosomal small nuclear ribonucleoproteins from Trypanosoma brucei.The EMBO journal · 2006Article
- trans and cis splicing in trypanosomatids: mechanism, factors, and regulation.Eukaryotic cell · 2003Review
- Assembly of the U1 snRNP involves interactions with the backbone of the terminal stem of U1 snRNA.RNA (New York, N.Y.) · 2003Article
- The spliced leader-associated RNA is a trypanosome-specific sn(o) RNA that has the potential to guide pseudouridine formation on the SL RNA.RNA (New York, N.Y.) · 2002Article
- Unconventional rules of small nuclear RNA transcription and cap modification in trypanosomatids.Gene expression · 2002Review
- The spliceosomal snRNP core complex of Trypanosoma brucei: cloning and functional analysis reveals seven Sm protein constituents.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Transcription termination and 3'-End processing of the spliced leader RNA in kinetoplastids.Molecular and cellular biology · 1999Article
- Exonic sequences in the 5' untranslated region of alpha-tubulin mRNA modulate trans splicing in Trypanosoma brucei.Molecular and cellular biology · 1998Article
- Three small nucleolar RNAs identified from the spliced leader-associated RNA locus in kinetoplastid protozoans.Molecular and cellular biology · 1998Article
- Structure-function analysis of the trypanosomatid spliced leader RNA.Nucleic acids research · 1998Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fractionation of the abundant small ribonucleoproteins (RNPs) of the trypanosomatid Leptomonas collosoma revealed the existence of a group of unidentified small RNPs that were shown to fractionate differently than the well-characterized trans-spliceosomal RNPs. One of these RNAs, an 80-nt RNA, did not possess a trimethylguanosine (TMG) cap structure but did possess a 5' phosphate terminus and an invariant consensus U5 snRNA loop 1. The gene coding for the RNA was cloned, and the coding region showed 55% sequence identity to the recently described U5 homologue of Trypanosoma brucei [Dungan, J. D., Watkins, K. P. & Agabian, N. (1996) EMBO J. 15, 4016-4029]. The L. collosoma U5 homologue exists in multiple forms of RNP complexes, a 10S monoparticle, and two subgroups of 18S particles that either contain or lack the U4 and U6 small nuclear RNAs, suggesting the existence of a U4/U6.U5 tri-small nuclear RNP complex. In contrast to T. brucei U5 RNA (62 nt), the L. collosoma homologue is longer (80 nt) and possesses a second stem-loop. Like the trypanosome U3, U6, and 7SL RNA genes, a tRNA gene coding for tRNACys was found 98 nt upstream to the U5 gene. A potential for base pair interaction between U5 and SL RNA in the 5' splice site region (positions -1 and +1) and downstream from it is proposed. The presence of a U5-like RNA in trypanosomes suggests that the most essential small nuclear RNPs are ubiquitous for both cis- and trans-splicing, yet even among the trypanosomatids the U5 RNA is highly divergent.
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