ArticleInfection and immunity1997
Complement fragment C5a and inflammatory cytokines in neutrophil recruitment during intramammary infection with Escherichia coli.
Article in Infection and immunity, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
48 citing papers in PubMed, 188 citations in OpenAlex.
- Feeding a high-concentrate corn straw diet increased the release of endotoxin in the rumen and pro-inflammatory cytokines in the mammary gland of dairy cows.BMC veterinary research · 2014Trial
- Milk cytokines and subclinical breast inflammation in Tanzanian women: effects of dietary red palm oil or sunflower oil supplementation.Immunology · 1999Trial
- LPS-Induced Intracellular Complement 3 Activation Regulated ATP Production in Yak Rumen Epithelial Cells.Veterinary sciences · 2025Article
- Relative expression of pro-inflammatory cytokine genes in Holstein dairy cows naturally affected byIranian journal of veterinary research · 2025Article
- Novel Approach for Evaluating Pregnancy-Associated Glycoprotein and Inflammation Markers during the Postpartum Period in Holstein Friesian Cows.Animals : an open access journal from MDPI · 2024Article
- Article
- Cytokine inflammatory response in dairy cows with mastitis caused byJournal of veterinary research · 2024Article
- Local and Systemic Effects ofMicroorganisms · 2023Review
- Colonization and local host response following intramammary Staphylococcus chromogenes challenge in dry cows.Veterinary research · 2021Article
- Efficacy of a Standardized Turmeric Extract Comprised of 70% Bisdemothoxy-Curcumin (REVERC3) Against LPS-Induced Inflammation in RAW264.7 Cells and Carrageenan-Induced Paw Edema.Journal of inflammation research · 2021Article
- Article
- A modular framework for multiscale, multicellular, spatiotemporal modeling of acute primary viral infection and immune response in epithelial tissues and its application to drug therapy timing and effectiveness.PLoS computational biology · 2020Article
- A modular framework for multiscale, multicellular, spatiotemporal modeling of acute primary viral infection and immune response in epithelial tissues and its application to drug therapy timing and effectiveness: A multiscale model of viral infection in epithelial tissues.bioRxiv : the preprint server for biology · 2020Article
- Potential roles of neutrophils in maintaining the health and productivity of dairy cows during various physiological and physiopathological conditions: a review.Immunologic research · 2019Review
- Subclinical endometritis in dairy cattle is associated with distinct mRNA expression patterns in blood and endometrium.PloS one · 2019Article
- Improved detection of biomarkers in cervico-vaginal mucus (CVM) from postpartum cattle.BMC veterinary research · 2018Article
- Cortisol modulates inflammatory responses in LPS-stimulated RAW264.7 cells via the NF-κB and MAPK pathways.BMC veterinary research · 2018Article
- Convergent and Divergent Migratory Patterns of Human Neutrophils inside Microfluidic Mazes.Scientific reports · 2018Article
- Sensing of Escherichia coli and LPS by mammary epithelial cells is modulated by O-antigen chain and CD14.PloS one · 2018Article
- A strategy to estimate the rate of recruitment of inflammatory cells during bovine intramammary infection under field management.BMC veterinary research · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Generation of inflammatory mediators and leukocyte recruitment to infection at an epithelial surface were studied during Escherichia coli-induced mastitis. One uninfected gland of each of eight midlactation cows was challenged with only 30 CFU of E. coli McDonald strain 487, a serum-resistant isolate from a cow with mastitis. Bacteria grew logarithmically during the first 10 to 12 h after challenge, reaching concentrations of more than 10(5) CFU/ml with no detectable host response during this time. An intense inflammatory reaction began approximately 12 h after the challenge and was characterized by a breakdown in the blood-milk permeability barrier followed by pyrexia and a pronounced leukocytic influx. Coincident with the onset of mammary inflammation was the appearance of neutrophil chemotactic activity in the milk from infected glands. Factors able to upregulate CD18 expression on peripheral blood neutrophils also appeared in milk at this time. The lack of appearance of chemotactic and CD18-upregulating activities until 12 h after challenge indicated that delays in neutrophil recruitment resulted from an initial lack of bacterial recognition and inflammatory mediator production. Production of complement fragment C5a, tumor necrosis factor, and interleukin-1 (IL-1) occurred earlier than production of IL-6 or IL-8. The early and intense production of C5a indicates that this chemoattractant may be more important than IL-8 during the initial recruitment and activation of neutrophils to a developing E. coli infection.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.