ArticleInfection and immunity1997
Activation of host cell protein kinase C by enteropathogenic Escherichia coli.
Article in Infection and immunity, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
33 citing papers in PubMed, 85 citations in OpenAlex.
- Observational
- EnterohemorrhagicFrontiers in cell and developmental biology · 2020Article
- Plumbagin Prevents Secretory Diarrhea by Inhibiting CaCC and CFTR Channel Activities.Frontiers in pharmacology · 2019Article
- Clever Cooperation: Interactions Between EspF and Host Proteins.Frontiers in microbiology · 2018Review
- Pathogen-induced secretory diarrhea and its prevention.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2016Review
- Pseudomonas aeruginosa Activates PKC-Alpha to Invade Middle Ear Epithelial Cells.Frontiers in microbiology · 2016Article
- Novel Host Proteins and Signaling Pathways in Enteropathogenic E. coli Pathogenesis Identified by Global Phosphoproteome Analysis.Molecular & cellular proteomics : MCP · 2015Article
- Pathogenesis of human enterovirulent bacteria: lessons from cultured, fully differentiated human colon cancer cell lines.Microbiology and molecular biology reviews : MMBR · 2013Review
- The enteropathogenic Escherichia coli-secreted protein EspZ inhibits host cell apoptosis.Infection and immunity · 2012Article
- Article
- Article
- Outer membrane protein A expression in Enterobacter sakazakii is required to induce microtubule condensation in human brain microvascular endothelial cells for invasion.Microbial pathogenesis · 2008Article
- Enteropathogenic E. coli-induced barrier function alteration is not a consequence of host cell apoptosis.American journal of physiology. Gastrointestinal and liver physiology · 2008Article
- Krüppel-like factor 5 mediates transmissible murine colonic hyperplasia caused by Citrobacter rodentium infection.Gastroenterology · 2008Article
- Mutual enhancement of virulence by enterotoxigenic and enteropathogenic Escherichia coli.Infection and immunity · 2006Article
- Enteropathogenic and enterohemorrhagic Escherichia coli infections: translocation, translocation, translocation.Infection and immunity · 2005Review
- Mouse model of enteropathogenic Escherichia coli infection.Infection and immunity · 2005Article
- Review
- Specific entry of Helicobacter pylori into cultured gastric epithelial cells via a zipper-like mechanism.Infection and immunity · 2002Article
- Induction of epithelial cell death including apoptosis by enteropathogenic Escherichia coli expressing bundle-forming pili.Infection and immunity · 2001Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Enteropathogenic Escherichia coli (EPEC) consists of a group of diarrhea-producing E. coli strains, common in developing countries, which do not produce classical toxins and are not truly invasive. EPEC strains adhere to mammalian cells in an intimate fashion, trigger a localized increase in intracellular calcium levels, and elevate inositol phosphate production. We hypothesized that these mediators could activate host cell protein kinase C (PKC) and tested this idea in vitro with two cultured human cell lines, HeLa cells and T84 cells. Using a recently described subculturing protocol to "induce" or accelerate EPEC adherence, we infected the cells with EPEC at a multiplicity of infection of approximately 100:1 for 30 to 60 min. Under these conditions, EPEC E2348 increased membrane-bound PKC activity 1.5- to 2.3-fold in HeLa cells and T84 cells, respectively. The increase in membrane-bound PKC activity was accompanied by a decrease in cytosolic PKC activity in EPEC-infected HeLa cells. Nonadherent laboratory E. coli strains such as HB101 and H.S. failed to trigger any consistent change in PKC production, similar to the nonadherent mutant strains derived from E2348, JPN15 (plasmid cured) and CVD206 (eaeA). In addition, immunoblots performed on extracts of T84 cells with a monoclonal antibody against PKC-alpha showed an increased PKC content in membranes of EPEC-infected cells. Finally, EPEC-infected T84 cells showed a 60% increase in responsiveness to the E. coli heat-stable toxin. We conclude that mediators produced in response to EPEC adherence activate PKC in intestinal and nonintestinal cells.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.