ArticleMolecular and cellular biology1997
CCAAT-binding factor NF-Y and RFX are required for in vivo assembly of a nucleoprotein complex that spans 250 base pairs: the invariant chain promoter as a model.
Article in Molecular and cellular biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Auto-fatty acylation of transcription factor RFX3 regulates ciliogenesis.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Regulation of cellulase and hemicellulase gene expression in fungi.Current genomics · 2013Article
- Identification and characterization of putative methylation targets in the MAOA locus using bioinformatic approaches.Epigenetics · 2010Article
- Metabolic engineering strategies for the improvement of cellulase production by Hypocrea jecorina.Biotechnology for biofuels · 2009Article
- Short Promoters in Viral Vectors Drive Selective Expression in Mammalian Inhibitory Neurons, but do not Restrict Activity to Specific Inhibitory Cell-Types.Frontiers in neural circuits · 2009Article
- DNA compaction by the nuclear factor-Y.Biophysical journal · 2007Article
- Transcriptional modulation of the pre-implantation embryo-specific Rnf35 gene by the Y-box protein NF-Y/CBF.The Biochemical journal · 2005Article
- Histone deacetylase 1/mSin3A disrupts gamma interferon-induced CIITA function and major histocompatibility complex class II enhanceosome formation.Molecular and cellular biology · 2003Article
- LEAFY COTYLEDON1-LIKE defines a class of regulators essential for embryo development.The Plant cell · 2003Article
- DEK binding to class II MHC Y-box sequences is gene- and allele-specific.Arthritis research & therapy · 2003Article
- Characterization of the human ephrin-A4 promoter.The Biochemical journal · 2002Article
- Major histocompatibility complex class II transcriptional platform: assembly of nuclear factor Y and regulatory factor X (RFX) on DNA requires RFX5 dimers.Molecular and cellular biology · 2002Article
- Self-association of CIITA and its transactivation potential.Molecular and cellular biology · 2001Article
- Endoplasmic reticulum stress-induced formation of transcription factor complex ERSF including NF-Y (CBF) and activating transcription factors 6alpha and 6beta that activates the mammalian unfolded protein response.Molecular and cellular biology · 2001Article
- Transcriptional scaffold: CIITA interacts with NF-Y, RFX, and CREB to cause stereospecific regulation of the class II major histocompatibility complex promoter.Molecular and cellular biology · 2000Article
- Sp1 mediates cell proliferation-dependent regulation of rat DNA topoisomerase IIalpha gene promoter.The Biochemical journal · 1999Article
- AnCF, the CCAAT binding complex of Aspergillus nidulans, is essential for the formation of a DNase I-hypersensitive site in the 5' region of the amdS gene.Molecular and cellular biology · 1999Article
- AnCF, the CCAAT binding complex of Aspergillus nidulans, contains products of the hapB, hapC, and hapE genes and is required for activation by the pathway-specific regulatory gene amdR.Molecular and cellular biology · 1999Article
- Interactions between the class II transactivator and CREB binding protein increase transcription of major histocompatibility complex class II genes.Molecular and cellular biology · 1999Article
- Transcriptional regulation of the MDR1 gene by histone acetyltransferase and deacetylase is mediated by NF-Y.Molecular and cellular biology · 1998Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
The events that lead to promoter accessibility within chromatin are not completely understood. The invariant chain (Ii) promoter was used as a model to determine the contribution of different DNA-binding factors in establishing occupancy of a complex promoter. Gamma interferon induction of the Ii promoter requires the cooperation of multiple cis elements including distal S, X, and Y/CCAAT elements along with proximal GC and Y/CCAAT elements. The heteromeric transcription factor NF-Y binds to both Y/CCAAT elements. Genomic footprinting was used to analyze in vivo protein-DNA contacts for integrated Ii promoters bearing mutations in each element. The results reveal a hierarchy of transcription factor loading with NF-Y binding to the distal Y/CCAAT element being required for establishing protein-DNA interactions over the entire 250 bp analyzed. Mutation of the X box disrupts binding primarily at the adjacent Y/CCAAT element along with a lesser effect on GC box binding. Importantly, this finding is verified with a cell line which lacks a functional X-box-binding factor, RFX, providing physiological validity for the strategy described here. Mutation of both the S element and the GC box results in either no or little effect on transcription factor binding. However, mutation of the proximal Y/CCAAT element disrupts binding to the adjacent GC box and partially reduces binding in the distal S/X/Y domain. The crucial role for NF-Y in establishing promoter occupancy may be related to its histone fold motif, the essential component for assembling nucleosome-like structures.
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