ArticleMolecular and cellular biology1997
Type I elements mediate replication fork pausing at conserved upstream sites in the Tetrahymena thermophila ribosomal DNA minichromosome.
Article in Molecular and cellular biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 33 citations in OpenAlex.
- Developmentally Programmed Switches in DNA Replication: Gene Amplification and Genome-Wide Endoreplication inMicroorganisms · 2023Article
- Ribosomal DNA instability and genome adaptability.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2019Review
- Review
- Checkpoint Activation of an Unconventional DNA Replication Program in Tetrahymena.PLoS genetics · 2015Article
- Developmental regulation of the Tetrahymena thermophila origin recognition complex.PLoS genetics · 2015Article
- Impaired replication elongation in Tetrahymena mutants deficient in histone H3 Lys 27 monomethylation.Genes & development · 2013Article
- Preferential re-replication of Drosophila heterochromatin in the absence of geminin.PLoS genetics · 2010Article
- Tetrahymena ORC contains a ribosomal RNA fragment that participates in rDNA origin recognition.The EMBO journal · 2007Article
- Replication fork stalling at natural impediments.Microbiology and molecular biology reviews : MMBR · 2007Review
- Deletion of the Tetrahymena thermophila rDNA replication fork barrier region disrupts macronuclear rDNA excision and creates a fragile site in the micronuclear genome.Nucleic acids research · 2006Article
- TIF1 Represses rDNA replication initiation, but promotes normal S phase progression and chromosome transmission in Tetrahymena.Molecular biology of the cell · 2005Article
- Coordination of replication and transcription along a Drosophila chromosome.Genes & development · 2004Article
- Characterization of a novel origin recognition complex-like complex: implications for DNA recognition, cell cycle control, and locus-specific gene amplification.Molecular and cellular biology · 2003Article
- Origin usage during euplotes ribosomal DNA amplification.Eukaryotic cell · 2003Article
- DNA replication forks pause at silent origins near the HML locus in budding yeast.Molecular and cellular biology · 2001Article
- Three different proteins recognize a multifunctional determinant that controls replication initiation, fork arrest and transcription in Tetrahymena.Nucleic acids research · 2000Article
- Long range cooperative interactions regulate the initiation of replication in the Tetrahymena thermophila rDNA minichromosome.Nucleic acids research · 1999Article
- The high mobility group protein Abf2p influences the level of yeast mitochondrial DNA recombination intermediates in vivo.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- A promoter region mutation affecting replication of the Tetrahymena ribosomal DNA minichromosome.Molecular and cellular biology · 1998Article
- Developmental regulation of DNA replication: replication fork barriers and programmed gene amplification in Tetrahymena thermophila.Molecular and cellular biology · 1997Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Two-dimensional gel electrophoresis was used to study replication of the Tetrahymena thermophila ribosomal DNA (rDNA) minichromosome. During vegetative growth, the rDNA is replicated exclusively from origins in the 5' nontranscribed spacer (NTS). Whereas replication fork movement through the rest of the chromosome appears to be continuous, movement through the 5' NTS is not. Replication forks arrest transiently at three prominent replication fork pausing sites (RFPs) located in or immediately adjacent to nucleosome-free regions of the 5' NTS. Pausing at these sites is dramatically diminished during replication in Escherichia coli, suggesting that chromatin organization or Tetrahymena-specific proteins may be required. A conserved tripartite sequence was identified at each pausing site. Mutations in type I elements diminish pausing at proximal RFPs. Hence, type I elements, previously shown to control replication initiation, also regulate elongation of existing replication forks. Studies with rDNA transformants revealed a strong directional bias for fork pausing. Strong pausing only occurred in forks moving toward the rRNA-coding region. We propose that fork pausing in the 5' NTS evolved to synchronize replication and transcription of the downstream rRNA genes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.