ArticleThe EMBO journal1997
Cells strongly expressing Ig(kappa) transgenes show clonal recruitment of hypermutation: a role for both MAR and the enhancers.
Article in The EMBO journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
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Who cites it
35 citing papers in PubMed, 109 citations in OpenAlex.
- Lamin B1 safeguards the B cell genome and shapes lymphoma outcome.HemaSphere · 2026Article
- The IgHFrontiers in immunology · 2023Article
- Transcription factor binding at Ig enhancers is linked to somatic hypermutation targeting.European journal of immunology · 2020Article
- Histone H3.3 promotes IgV gene diversification by enhancing formation of AID-accessible single-stranded DNA.The EMBO journal · 2016Article
- Targeting of somatic hypermutation by immunoglobulin enhancer and enhancer-like sequences.PLoS biology · 2014Article
- AID and Apobec3G haphazard deamination and mutational diversity.Cellular and molecular life sciences : CMLS · 2013Review
- Multiple transcription factor binding sites predict AID targeting in non-Ig genes.Journal of immunology (Baltimore, Md. : 1950) · 2013Article
- AID targeting is dependent on RNA polymerase II pausing.Seminars in immunology · 2012Review
- Stabilised DNA secondary structures with increasing transcription localise hypermutable bases for somatic hypermutation in IGHV3-23.Immunogenetics · 2012Article
- A coming-of-age story: activation-induced cytidine deaminase turns 10.Nature immunology · 2009Review
- PU.1 can recruit BCL6 to DNA to repress gene expression in germinal center B cells.Molecular and cellular biology · 2009Article
- Control of gene conversion and somatic hypermutation by immunoglobulin promoter and enhancer sequences.The Journal of experimental medicine · 2006Article
- The in vivo pattern of AID targeting to immunoglobulin switch regions deduced from mutation spectra in msh2-/- ung-/- mice.The Journal of experimental medicine · 2006Article
- AID to overcome the limitations of genomic information by introducing somatic DNA alterations.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2006Review
- Antibody diversification: mutational mechanisms and oncogenesis.Immunologic research · 2006Review
- Complex regulation of somatic hypermutation by cis-acting sequences in the endogenous IgH gene in hybridoma cells.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Hepatitis C virus E2-CD81 interaction induces hypermutation of the immunoglobulin gene in B cells.Journal of virology · 2005Article
- Genome-wide somatic hypermutation.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Somatic hypermutation is limited by CRM1-dependent nuclear export of activation-induced deaminase.The Journal of experimental medicine · 2004Article
- Hepatitis C virus induces a mutator phenotype: enhanced mutations of immunoglobulin and protooncogenes.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The V regions of immunoglobulin kappa transgenes are targets for hypermutation in germinal centre B cells. We show by use of modified transgenes that the recruitment of hypermutation is substantially impaired by deletion of the nuclear matrix attachment region (MAR) which flanks the intron-enhancer (Ei). Decreased mutation is also obtained if Ei, the core region of the kappa3'-enhancer (E3') or the E3'-flank are removed individually. A broad correlation between expression and mutation is indicated not only by the fact that the deletions affecting mutation also give reduced transgene expression, but especially by the finding that, within a single mouse, transgene mutation was considerably reduced in germinal centre B cells that poorly expressed the transgene as compared with strongly expressing cells. We also observed that the diminished mutation in transgenes carrying regulatory element deletions was manifested by an increased proportion of B cells in which the transgene had not been targeted at all for mutation rather than in the extent of mutation accumulation once targeted. Since mutations appear to be incorporated stepwise, the results point to a connection between transcription initiation and the clonal recruitment of hypermutation, with hypermutation being more fastidious than transcription in requiring the presence of a full complement of regulatory elements.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.