ArticleThe EMBO journal1997
ZEB, a vertebrate homolog of Drosophila Zfh-1, is a negative regulator of muscle differentiation.
Article in The EMBO journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT01480141 (A Therapeutic Trial of Afatinib in the Neoadjuvant Setting. Molecular Effects of Afatinib), which is not on this map. Cited by 66 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
A Therapeutic Trial of Afatinib in the Neoadjuvant Setting. Molecular Effects of Afatinib: A Window of Opportunity Trial In Early Stage NSCLC
Who cites it
66 citing papers in PubMed, 143 citations in OpenAlex.
- Nuclear gasdermin E drives endothelin-1-induced metastatic progression independently of the pyroptosis.Cell death & disease · 2026Article
- How ATP-Dependent Chromatin Remodeling Complexes Regulate Vertebrate Embryonic Development.International journal of molecular sciences · 2026Review
- Epithelial-to-mesenchymal transition transcription factors: New strategies for mesenchymal tissue regeneration.Cytokine & growth factor reviews · 2025Review
- Two sequential gene expression programs bridged by cell division support long-distance collective cell migration.Development (Cambridge, England) · 2024Article
- Article
- The transcription factor Zfh1 acts as a wing-morph switch in planthoppers.Nature communications · 2022Article
- Mechanisms of Binding Specificity among bHLH Transcription Factors.International journal of molecular sciences · 2021Review
- Zeb1 modulates hematopoietic stem cell fates required for suppressing acute myeloid leukemia.The Journal of clinical investigation · 2021Article
- Targeting endothelin 1 receptor-miR-200b/c-ZEB1 circuitry blunts metastatic progression in ovarian cancer.Communications biology · 2020Article
- Zeb2 Regulates Myogenic Differentiation in Pluripotent Stem Cells.International journal of molecular sciences · 2020Article
- ZEB1 protects skeletal muscle from damage and is required for its regeneration.Nature communications · 2019Article
- New Insights into the Role of Epithelial⁻Mesenchymal Transition during Aging.International journal of molecular sciences · 2019Review
- Regulation of muscle atrophy-related genes by the opposing transcriptional activities of ZEB1/CtBP and FOXO3.Nucleic acids research · 2018Article
- Zeb1 potentiates genome-wide gene transcription with Lef1 to promote glioblastoma cell invasion.The EMBO journal · 2018Article
- ZEB Proteins in Leukemia: Friends, Foes, or Friendly Foes?HemaSphere · 2018Review
- Tumor-associated macrophages (TAMs) depend on ZEB1 for their cancer-promoting roles.The EMBO journal · 2017Article
- Article
- Fat deposition and accumulation in the damaged and inflamed skeletal muscle: cellular and molecular players.Cellular and molecular life sciences : CMLS · 2015Review
- Article
- Article
6 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A number of genes, spanning the evolutionary scale from yeast to mammals, that are involved in spatial and temporal patterning during development contain zinc finger and homeodomain motifs. One such zinc finger/homeodomain protein is Drosophila Zfh-1, a member of the zfh family of Drosophila genes, which is expressed in muscle precursors and is critical for the proper development of muscle. Here we demonstrate that a vertebrate homolog of Zfh-1 (ZEB) is a negative regulator of muscle differentiation. We show that ZEB binds to a subset of E boxes in muscle genes and functions by actively repressing transcription. One target of this repression is the members of the MEF-2 family, which synergize with proteins of the myogenic basic helix-loop-helix family (bHLH) (myoD, myf-5, myogenin and MRF-4) to induce myogenic differentiation. As muscle differentiation proceeds, myogenic bHLH proteins accumulate to levels sufficient to displace ZEB from the E boxes, releasing the repression and allowing bHLH proteins to further activate transcription. This mechanism of active transcriptional repression distinguishes ZEB from other negative regulators of myogenesis (Id, Twist and I-mfa) that inhibit muscle differentiation by simply binding and inactivating myogenic factors. The relative affinity of ZEB versus myogenic bHLH proteins varies for E boxes in different genes such that ZEB would be displaced from different genes at distinct times as myogenic bHLH proteins accumulate during myogenesis, thus providing a mechanism to regulate temporal order of gene expression.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.