ArticleThe EMBO journal1997
Specific arginine and threonine residues control anion binding and transport in the light-driven chloride pump halorhodopsin.
Article in The EMBO journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 54 citations in OpenAlex.
- Functional Mechanism of ClAdvances in experimental medicine and biology · 2021Review
- Structure of a retinal chromophore of dark-adapted middle rhodopsin as studied by solid-state nuclear magnetic resonance spectroscopy.Biophysics and physicobiology · 2021Article
- The crystal structures of a chloride-pumping microbial rhodopsin and its proton-pumping mutant illuminate proton transfer determinants.The Journal of biological chemistry · 2020Article
- Crystal structure of Halobacterium salinarum halorhodopsin with a partially depopulated primary chloride-binding site.Acta crystallographica. Section F, Structural biology communications · 2016Article
- Getting it through your thick skull.Nature neuroscience · 2014Article
- Noninvasive optical inhibition with a red-shifted microbial rhodopsin.Nature neuroscience · 2014Article
- Ser(262) determines the chloride-dependent colour tuning of a new halorhodopsin from Haloquadratum walsbyi.Bioscience reports · 2012Article
- Genetically encoded molecular tools for light-driven silencing of targeted neurons.Progress in brain research · 2012Review
- A history of optogenetics: the development of tools for controlling brain circuits with light.F1000 biology reports · 2011Article
- Synthetic physiology strategies for adapting tools from nature for genetically targeted control of fast biological processes.Methods in enzymology · 2011Article
- Heterologous expression of Pharaonis halorhodopsin in Xenopus laevis oocytes and electrophysiological characterization of its light-driven Cl- pump activity.Biophysical journal · 2007Article
- Chlamydomonas reinhardtii strains expressing nitrate reductase under control of the cabII-1 promoter: isolation of chlorate resistant mutants and identification of new loci for nitrate assimilation.Photosynthesis research · 2005Article
- Cl(-) concentration dependence of photovoltage generation by halorhodopsin from Halobacterium salinarum.Biophysical journal · 2002Article
- Temperature and halide dependence of the photocycle of halorhodopsin from Natronobacterium pharaonis.Biophysical journal · 2001Article
- Roles of cytoplasmic arginine and threonine in chloride transport by the bacteriorhodopsin mutant D85T.Biophysical journal · 2001Article
- Halide dependence of the halorhodopsin photocycle as measured by time-resolved infrared spectra.Biophysical journal · 2001Article
- Role of cationic amino acids in the Na+/dicarboxylate co-transporter NaDC-1.The Biochemical journal · 2000Article
- Charge motions during the photocycle of pharaonis halorhodopsin.Biophysical journal · 2000Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The light-driven chloride pump halorhodopsin (HR), a halobacterial retinal protein, was studied by comparing wild type with specific mutants. Changes of conserved arginine and threonine residues in the transmembrane regions could be classified in two categories: in the extracellular half of the molecule, mutations influence anion uptake and binding. R108 mutations abolish all anion effects previously attributed to two distinct binding sites and change the characteristic photochemistry. Neutral residues at position 108 completely inactivate the pump. T111 increases the affinity of this anion binding site without being essentially important. In the photochemical cycles of the mutants T111V and Q105E, a red-shifted absorbing intermediate is enriched indicating retarded anion uptake. On the cytoplasmic side, mutations do not change anion binding properties of the unphotolyzed protein, but slow down anion release thereby reducing the chloride transport activity and the photocycling rate. The lowest activity is found for T203V, while R200 mutations have weaker effects. Thus, in the symmetrically arranged pairs R108/T111 and T203/R200, threonine and arginine play different roles, reflecting high affinity anion uptake by the former and effective anion release catalyzed by the latter residues. A model for the anion transport mechanism in HR is suggested comprising the specific functions of channel-lining residues.
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