ArticleTransgenic research1997
Recombinant human extracellular superoxide dismutase produced in milk of transgenic rabbits.
Article in Transgenic research, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed, 45 citations in OpenAlex.
- Production of functional human CuZn-SOD and EC-SOD in bitransgenic cloned goat milk.Transgenic research · 2018Article
- Recombinant human plasma phospholipid transfer protein (PLTP) to prevent bacterial growth and to treat sepsis.Scientific reports · 2017Article
- Expression systems and species used for transgenic animal bioreactors.BioMed research international · 2013Article
- Expression of human growth hormone in the milk of transgenic rabbits with transgene mapped to the telomere region of chromosome 7q.Journal of applied genetics · 2012Article
- Aerosolized human extracellular superoxide dismutase prevents hyperoxia-induced lung injury.PloS one · 2011Article
- Could protein tertiary structure influence mammary transgene expression more than tissue specific codon usage?Transgenic research · 2010Article
- Chromosomal localization of a proinsulin transgene in Japanese quail by laser pressure catapulting.Transgenic research · 2006Article
- The transgenic rabbit as model for human diseases and as a source of biologically active recombinant proteins.Transgenic research · 2003Review
- Transgenic animal bioreactors.Transgenic research · 2000Review
- Transgenic milk as a method for the production of recombinant antibodies.Journal of immunological methods · 1999Review
- Stable production of human insulin-like growth factor 1 (IGF-1) in the milk of hemi- and homozygous transgenic rabbits over several generations.Transgenic research · 1998Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Expression of human extracellular superoxide dismutase (EC-SOD), a glycosylated, tetrameric metalloprotein, was targeted to the lactating mammary gland of transgenic rabbits. Efficient expression of the recombinant whey acidic protein/ec-sod gene was achieved and up to 3 mg ml-1 of the enzyme was secreted into the milk. Rabbit milk-produced recombinant EC-SOD was primarily found in the whey and purified by a two-step chromatographic method. To evaluate the rabbit milk-produced human EC-SOD, comparisons with native and Chinese hamster ovary cell (CHO)-produced EC-SOD were performed. All proteins were tetrameric and N-glycosylated. The behaviour on SDS-PAGE and size-exclusion chromatography indicated that the masses, and thereby the extent of post-translational modification of the proteins was similar. The monosaccharide composition of both recombinant EC-SOD variants was analysed and indicated similarities in the attached N-glycans on the two proteins. Furthermore, the peptide maps of the three EC-SOD variants revealed that all proteins had similar polypeptide backbones.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.