ArticleJournal of clinical microbiology1997
Application of a nested, multiplex PCR to psittacosis outbreaks.
Article in Journal of clinical microbiology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 28 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Healthy animals, healthy people: zoonosis risk from animal contact in pet shops, a systematic review of the literature.PloS one · 2014Pooled it
- Molecular Detection and Genotyping ofAnimals : an open access journal from MDPI · 2024Article
- First detection of Chlamydia avium in healthy Amazon parrots (Amazona aestiva) in Argentina.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2023Article
- Chlamydia psittaci in Faecal Samples of Feral Pigeons (Columba livia forma urbana) in Urban Areas of Lublin city, Poland.Current microbiology · 2022Article
- The Current State of Knowledge on Baggio-Yoshinari Syndrome (Brazilian Lyme Disease-like Illness): Chronological Presentation of Historical and Scientific Events Observed over the Last 30 Years.Pathogens (Basel, Switzerland) · 2022Review
- Detection of Chlamydial DNA from Mediterranean Loggerhead Sea Turtles in Southern Italy.Animals : an open access journal from MDPI · 2022Article
- Development of a Lateral Flow Strip-Based Recombinase-Aided Amplification for ActiveFrontiers in microbiology · 2022Article
- Reliable and Sensitive Nested PCR for the Detection ofMicroorganisms · 2021Article
- A recombinase polymerase amplification-based assay for rapid detection of Chlamydia psittaci.Poultry science · 2021Article
- FatalJournal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2021Article
- Establishment of a Multiplex Real-Time TaqMan-MGB Polymerase Chain Reaction (PCR) Method for the Simultaneous Detection of Three Animal Chlamydia Species.Medical science monitor : international medical journal of experimental and clinical research · 2019Article
- An outbreak of psittacosis at a veterinary school demonstrating a novel source of infection.One health (Amsterdam, Netherlands) · 2017Article
- Clinical features of endemic community-acquired psittacosis.New microbes and new infections · 2014Article
- Chlamydia psittaci infection in canaries heavily infested by Dermanyssus gallinae.Experimental & applied acarology · 2011Article
- An economical tandem multiplex real-time PCR technique for the detection of a comprehensive range of respiratory pathogens.Viruses · 2009Article
- Real-time PCR detection and quantitation of Chlamydophila psittaci in human and avian specimens from a veterinary clinic cluster.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2008Article
- A novel nested multiplex polymerase chain reaction (PCR) assay for differential detection of Entamoeba histolytica, E. moshkovskii and E. dispar DNA in stool samples.BMC microbiology · 2007Article
- Use of a nested PCR-enzyme immunoassay with an internal control to detect Chlamydophila psittaci in turkeys.BMC infectious diseases · 2005Article
- SARS surveillance during emergency public health response, United States, March-July 2003.Emerging infectious diseases · 2004Article
- Specificity and performance of PCR detection assays for microbial pathogens.Molecular biotechnology · 2004Review
Corrections and comments
- Erratum issued
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We developed a nested, multiplex PCR for simultaneous detection of three species of chlamydiae in human and avian specimens. The PCR was designed to increase sensitivity and to circumvent inhibitors of PCR present in clinical specimens. The target sequence was the 16S rRNA gene. The first-step PCR was genus specific, and the second-step PCR was multiplexed (i.e., had multiple primer sets in the same tube) and could discriminate among Chlamydia pneumoniae, Chlamydia psittaci, and Chlamydia trachomatis on the basis of the molecular weight of the amplicon. The limit of detection of each of the two PCR steps was 5 inclusion-forming units. We used PCR and serologic evidence during outbreaks of psittacosis to infer that C. psittaci had been transmitted from birds purchased in pet stores to humans. We also used this method to test both live and dead birds from pet stores for infection with C. psittaci. Compared with culture, the application of PCR to avian specimens increased the rate of C. psittaci detection.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.