ArticleJournal of clinical microbiology1997
Identification of Candida species by randomly amplified polymorphic DNA fingerprinting of colony lysates.
Article in Journal of clinical microbiology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Improved RAPD Method forGenes · 2023Article
- Budding off: bringing functional genomics to Candida albicans.Briefings in functional genomics · 2016Review
- Usefulness of McRAPD for typing and importance of biofilm production in a case of nosocomial ventriculoperitoneal shunt infection caused by Candida lusitaniae.Folia microbiologica · 2011Article
- Performance of optimized McRAPD in identification of 9 yeast species frequently isolated from patient samples: potential for automation.BMC microbiology · 2009Article
- Differentiation of Aspergillus niger by random amplification of polymorphic DNA.Journal of industrial microbiology & biotechnology · 2008Article
- Primer R108 performs best in the RAPD strain typing of three Aspergillus species frequently isolated from patients.Folia microbiologica · 2006Article
- Phenotypic and molecular characterization of Candida nivariensis sp. nov., a possible new opportunistic fungus.Journal of clinical microbiology · 2005Article
- Identification of Streptococcus sanguinis with a PCR-generated species-specific DNA probe.Journal of clinical microbiology · 2003Article
- Identification of Candida spp. by randomly amplified polymorphic DNA analysis and differentiation between Candida albicans and Candida dubliniensis by direct PCR methods.Journal of clinical microbiology · 2003Article
- Multiplex PCR using internal transcribed spacer 1 and 2 regions for rapid detection and identification of yeast strains.Journal of clinical microbiology · 2001Article
- Antifungal effects of lysozyme and lactoferrin against genetically similar, sequential Candida albicans isolates from a human immunodeficiency virus-infected southern Chinese cohort.Journal of clinical microbiology · 2001Article
- Candidiasis during pregnancy may result from isogenic commensal strains.Infectious diseases in obstetrics and gynecology · 2001Article
- Molecular characterization of Yarrowia lipolytica and Candida zeylanoides isolated from poultry.Applied and environmental microbiology · 2000Article
- Oral colonization, phenotypic, and genotypic profiles of Candida species in irradiated, dentate, xerostomic nasopharyngeal carcinoma survivors.Journal of clinical microbiology · 2000Article
- The ins and outs of DNA fingerprinting the infectious fungi.Clinical microbiology reviews · 2000Review
- PCR assay for species-specific identification of Bacteroides thetaiotaomicron.Journal of clinical microbiology · 2000Article
- High-frequency, in vitro reversible switching of Candida lusitaniae clinical isolates from amphotericin B susceptibility to resistance.Antimicrobial agents and chemotherapy · 1999Article
- Candida glabrata: review of epidemiology, pathogenesis, and clinical disease with comparison to C. albicans.Clinical microbiology reviews · 1999Review
- Stable phenotypic resistance of Candida species to amphotericin B conferred by preexposure to subinhibitory levels of azoles.Journal of clinical microbiology · 1998Article
- Identification and Antifungal Susceptibility Testing ofJournal of global infectious diseasesArticle
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have characterized a method that produces simple yet diagnostic fingerprints that are unique to isolates of Candida species. DNA from individual colonies can be amplified from crude single-colony lysates. Randomly amplified polymorphic DNA (RAPD) fingerprints generated from a single primer correctly identified the species of most (>98%) of the isolates identified with CHROMagar Candida plates as non-Candida albicans Candida species. RAPD fingerprints were much more informative than the plates, since they distinguished between all tested species and required less time. Most (91%) of these identifications agreed with those assigned by API 20C tests. In almost every incident of species identity mismatch, electrophoretic karyotyping showed that the RAPD fingerprint was correct. This underscores the improved objectivity and reliability of this method over those of conventional diagnostic tools. The identities of approximately 30% of C. albicans isolates identified in clinical laboratories by positive germ tube tests are not verified by either testing on CHROMagar Candida plates or RAPD fingerprinting. Data suggest that clinical isolates conventionally identified as C. albicans in clinical settings are heterogeneous, consisting of both misidentified and atypical yeasts. RAPD fingerprints obtained from primary culture plate colonies allows for rapid, highly accurate determinations of Candida species, hence permitting earlier selection of appropriate antifungal agents in the clinical setting.
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