ArticleThe Biochemical journal1997
Platelet-derived growth factor activation of mitogen-activated protein kinase depends on the sequential activation of phosphatidylcholine-specific phospholipase C, protein kinase C-zeta and Raf-1.
Article in The Biochemical journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 67 citations in OpenAlex.
- Machine learning approaches reveal methylation signatures associated with pediatric acute myeloid leukemia recurrence.Scientific reports · 2025Article
- Prevention of Venous Neointimal Hyperplasia by a Multitarget Receptor Tyrosine Kinase Inhibitor.Journal of vascular research · 2015Article
- Regulation of polarized morphogenesis by protein kinase C iota in oncogenic epithelial spheroids.Carcinogenesis · 2014Article
- Phosphatidylethanolamine binding protein 1 in vacular endothelial cell autophagy and atherosclerosis.The Journal of physiology · 2013Article
- Mice lacking the Raf-1 kinase inhibitor protein exhibit exaggerated hypoxia-induced pulmonary hypertension.British journal of pharmacology · 2011Article
- Silica induces macrophage cytokines through phosphatidylcholine-specific phospholipase C with hydrogen peroxide.American journal of respiratory cell and molecular biology · 2007Article
- Measurements of phospholipases A2, C, and D (PLA2, PLC, and PLD). In vitro microassays, analysis of enzyme isoforms, and intact-cell assays.Methods in molecular biology (Clifton, N.J.) · 2003Article
- Regulation of phospholipase D isoenzymes by transforming Ras and atypical protein kinase C-iota.The Biochemical journal · 2001Article
- MEK5, a new target of the atypical protein kinase C isoforms in mitogenic signaling.Molecular and cellular biology · 2001Article
- Different protein kinase C isoforms determine growth factor specificity in neuronal cells.Molecular and cellular biology · 2000Article
- Mapping of atypical protein kinase C within the nerve growth factor signaling cascade: relationship to differentiation and survival of PC12 cells.Molecular and cellular biology · 2000Article
- Protein kinase C activation by acidic proteins including 14-3-3.The Biochemical journal · 2000Article
- Protein kinase C zeta isoform is critical for proliferation in human glioblastoma cell lines.Journal of neuro-oncology · 2000Article
- 14-3-3 isotypes facilitate coupling of protein kinase C-zeta to Raf-1: negative regulation by 14-3-3 phosphorylation.The Biochemical journal · 2000Article
- p70 S6 kinase is regulated by protein kinase Czeta and participates in a phosphoinositide 3-kinase-regulated signalling complex.Molecular and cellular biology · 1999Article
- Atypical protein kinase Clambda binds and regulates p70 S6 kinase.The Biochemical journal · 1998Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The mechanism of Raf-1 activation by platelet-derived growth factor (PDGF) is poorly defined. We previously reported that, in Rat-1 fibroblasts, PDGF activates a phosphatidylcholine-specific phospholipase C (PC-PLC) and that the product, diacylglycerol, somehow activates protein kinase C-zeta (PKC-zeta). Both PC-PLC and PKC-zeta activities were required for PDGF activation of mitogen-activated protein kinase (MAPK). Now we report that MAPK activation by exogenous PC-PLC depends on Raf-1 activation. PKC-zeta co-immunoprecipitates with, phoshorylates and activates Raf-1, suggesting that in the PDGF- and PC-PLC-activated MAPK pathway, PKC-zeta operates in a signalling complex as a direct activator of Raf-1.
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