ArticleThe Journal of cell biology1997
CDK1 inactivation regulates anaphase spindle dynamics and cytokinesis in vivo.
Article in The Journal of cell biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 69 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
69 citing papers in PubMed, 162 citations in OpenAlex.
- The Effect ofLife (Basel, Switzerland) · 2025Article
- Anaphase B: Long-standing models meet new concepts.Seminars in cell & developmental biology · 2021Review
- Phosphorylation in the intrinsically disordered region of F-BAR protein Imp2 regulates its contractile ring recruitment.Journal of cell science · 2021Article
- Cdk1 phosphorylation of fission yeast paxillin inhibits its cytokinetic ring localization.Molecular biology of the cell · 2021Article
- Cytokinesis regulators as potential diagnostic and therapeutic biomarkers for human hepatocellular carcinoma.Experimental biology and medicine (Maywood, N.J.) · 2021Review
- Cell cycle oscillators underlying orderly proteolysis of E2F8.Molecular biology of the cell · 2020Article
- A Structural View on ESCRT-Mediated Abscission.Frontiers in cell and developmental biology · 2020Review
- Manipulating cultured mammalian cells for mitosis research.Methods in cell biology · 2020Article
- Article
- lncRNA Expression Reveals the Potential Regulatory Roles in Hepatocyte Proliferation during Rat Liver Regeneration.BioMed research international · 2019Article
- Cell-intrinsic and -extrinsic mechanisms promote cell-type-specific cytokinetic diversity.eLife · 2018Article
- Cdk1-dependent phosphoinhibition of a formin-F-BAR interaction opposes cytokinetic contractile ring formation.Molecular biology of the cell · 2018Article
- Human CDK18 promotes replication stress signaling and genome stability.Nucleic acids research · 2016Article
- Control of regional decidualization in implantation: Role of FoxM1 downstream of Hoxa10 and cyclin D3.Scientific reports · 2015Article
- Feedback control of chromosome separation by a midzone Aurora B gradient.Science (New York, N.Y.) · 2014Article
- Inefficient degradation of cyclin B1 re-activates the spindle checkpoint right after sister chromatid disjunction.Cell cycle (Georgetown, Tex.) · 2014Article
- A knockout screen for protein kinases required for the proper meiotic segregation of chromosomes in the fission yeast Schizosaccharomyces pombe.Cell cycle (Georgetown, Tex.) · 2013Article
- Plasmodium falciparum inhibitor-3 homolog increases protein phosphatase type 1 activity and is essential for parasitic survival.The Journal of biological chemistry · 2012Article
- Phosphorylation of a mitotic kinesin-like protein and a MAPKKK by cyclin-dependent kinases (CDKs) is involved in the transition to cytokinesis in plants.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Ipl1/Aurora-dependent phosphorylation of Sli15/INCENP regulates CPC-spindle interaction to ensure proper microtubule dynamics.The Journal of cell biology · 2011Article
9 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Through association with CDK1, cyclin B accumulation and destruction govern the G2/M/G1 transitions in eukaryotic cells. To identify CDK1 inactivation-dependent events during late mitosis, we expressed a nondestructible form of cyclin B (cyclin BDelta90) by microinjecting its mRNA into prometaphase normal rat kidney cells. The injection inhibited chromosome decondensation and nuclear envelope formation. Chromosome disjunction occurred normally, but anaphase-like movement persisted until the chromosomes reached the cell periphery, whereupon they often somersaulted and returned to the cell center. Injection of rhodamine-tubulin showed that this movement occurred in the absence of a central anaphase spindle. In 82% of cells cytokinesis was inhibited; the remainder split themselves into two parts in a process reminiscent of Dictyostelium cytofission. In all cells injected, F-actin and myosin II were diffusely localized with no detectable organization at the equator. Our results suggest that a primary effect of CDK1 inactivation is on spindle dynamics that regulate chromosome movement and cytokinesis. Prolonged CDK1 activity may prevent cytokinesis through inhibiting midzone microtubule formation, the behavior of proteins such as TD60, or through the phosphorylation of myosin II regulatory light chain.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.