ArticleJournal of virology1997
Attenuation of a human rotavirus vaccine candidate did not correlate with mutations in the NSP4 protein gene.
Article in Journal of virology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 50 citations in OpenAlex.
- Article
- Mutations of two amino acids in VP5 mediate the attenuation of human rotavirus vaccine: evidence fromJournal of virology · 2025Article
- Generation of Recombinant Authentic Live Attenuated Human Rotavirus Vaccine Strain RIX4414 (RotarixViruses · 2024Article
- Serial Passaging of the Human Rotavirus CDC-9 Strain in Cell Culture Leads to Attenuation: Characterization fromJournal of virology · 2020Article
- Virulence-associated genome mutations of murine rotavirus identified by alternating serial passages in mice and cell cultures.Journal of virology · 2014Article
- Article
- Conformational Differences Unfold a Wide Range of Enterotoxigenic Abilities Exhibited by rNSP4 Peptides from Different Rotavirus Strains.The open virology journal · 2011Article
- Rotavirus infection is not associated with small intestinal fluid secretion in the adult mouse.Journal of virology · 2006Article
- N- and C-terminal cooperation in rotavirus enterotoxin: novel mechanism of modulation of the properties of a multifunctional protein by a structurally and functionally overlapping conformational domain.Journal of virology · 2006Article
- Evidence for genetic linkage between the gene segments encoding NSP4 and VP6 proteins in common and reassortant human rotavirus strains.Journal of clinical microbiology · 2003Article
- I, 2. Physiology and pathophysiology of the gut in relation to viral diarrhea.Perspectives in medical virology · 2003Article
- A human vaccine strain of lamb rotavirus (Chinese) NSP4 gene: complete nucleotide sequence and phylogenetic analyses.Virus genes · 2003Article
- Diarrhea-inducing activity of avian rotavirus NSP4 glycoproteins, which differ greatly from mammalian rotavirus NSP4 glycoproteins in deduced amino acid sequence in suckling mice.Journal of virology · 2002Article
- NSP4 gene analysis of rotaviruses recovered from infected children with and without diarrhea.Journal of clinical microbiology · 2000Article
- Comparisons of nucleotide and deduced amino acid sequences of NSP4 genes of virulent and attenuated pairs of group A and C rotaviruses.Virus genes · 1999Article
- Mutations in rotavirus nonstructural glycoprotein NSP4 are associated with altered virus virulence.Journal of virology · 1998Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The NSP4 protein of a simian rotavirus was reported to induce diarrhea following inoculation of mice. If NSP4 is responsible for rotavirus diarrhea in humans, attenuation of a human rotavirus may be reflected in concomitant mutations in the NSP4 gene. After 33 passages in cultured monkey kidney cells, a virulent human rotavirus (strain 89-12) was found to be attenuated in adults, children, and infants. Nucleotide sequence analysis of the NSP4 protein gene revealed only one base pair change between the virulent (unpassaged) and attenuated 89-12 viruses, which resulted from a substitution of alanine for threonine at amino acid 45 of the encoded NSP4 protein. Because both threonine and alanine have been found at position 45 of NSP4 in symptomatic and asymptomatic human rotaviruses, neither amino acid in this position could be established as a marker of virulence. Therefore, attenuation of rotavirus strain 89-12 appears to be unrelated to mutations in the NSP4 gene.
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