ArticleProceedings of the National Academy of Sciences of the United States of America1997
Nontarget DNA sequences reduce the transgene length necessary for RNA-mediated tospovirus resistance in transgenic plants.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 92 citations in OpenAlex.
- Article
- Review
- Development of transgenic sweet potato with multiple virus resistance in South Africa (SA).Transgenic research · 2014Article
- Differential expression of tomato spotted wilt virus-derived viral small RNAs in infected commercial and experimental host plants.PloS one · 2013Article
- Development of transgenic watermelon resistant to Cucumber mosaic virus and Watermelon mosaic virus by using a single chimeric transgene construct.Transgenic research · 2012Article
- Article
- Evaluation of DNA fragments covering the entire genome of a monopartite begomovirus for induction of viral resistance in transgenic plants via gene silencing.Transgenic research · 2012Article
- Resistance to a DNA and a RNA virus in transgenic plants by using a single chimeric transgene construct.Transgenic research · 2011Article
- A transformation booster sequence (TBS) from Petunia hybrida functions as an enhancer-blocking insulator in Arabidopsis thaliana.Plant cell reports · 2009Article
- Matrix attachment regions increase the efficiency and stability of RNA-mediated resistance to tomato spotted wilt virus in transgenic tobacco.Transgenic research · 2005Article
- Transgenic or plant expression vector-mediated recombination of Plum Pox Virus.Journal of virology · 2000Article
- Phylogenetic analysis of two potyvirus pathogens of commercial cowpea lines: implications for obtaining pathogen-derived resistance.Virus genes · 2000Article
- Expression and sequence requirements for nitrite reductase co-suppression.Plant molecular biology · 1999Article
- Turnip mosaic potyvirus resistance in Nicotiana benthamiana derived by post-transcriptional gene silencing.Transgenic research · 1999Article
- Isolation of an RNA-directed RNA polymerase-specific cDNA clone from tomato.The Plant cell · 1998Article
- A model for RNA-mediated gene silencing in higher plants.Plant molecular biology · 1998Review
- Sequence characteristics of natural populations of tomato spotted wilt tospovirus infecting flue-cured tobacco in Georgia.Virus genes · 1998Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RNA-mediated virus resistance has recently been shown to be the result of post-transcriptional transgene silencing in transgenic plants. This study was undertaken to characterize the effect of transgene length and nontarget DNA sequences on RNA-mediated tospovirus resistance in transgenic plants. Transgenic Nicotiana benthamiana plants were generated to express different regions of the nucleocapsid (N) protein of tomato spotted wilt (TSWV) tospovirus. Transgenic plants expressing half-gene segments (387-453 bp) of the N gene displayed resistance through post-transcriptional gene silencing. Although smaller N gene segments (92-235 bp) were ineffective in conferring resistance when expressed alone in transgenic plants, these segments conferred resistance when fused to the nontarget green fluorescent protein gene DNA. These results demonstrate that (i) a critical length of N transgene (236-387 bp) is required for a high level of transgene expression and consequent gene silencing, and (ii) the post-transcriptional gene silencing mechanism can trans-inactivate the incoming tospovirus genome with homologous transgene segments that are as short as 110 bp. Therefore, the activation of post-transcriptional transgene silencing requires a significantly larger transgene than is required for the trans-inactivation of the incoming viral genome. These results raise the possibility of developing a simple new strategy for engineering multiple virus resistance in transgenic plants.
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