ArticleProceedings of the National Academy of Sciences of the United States of America1997
Transitions in the coupling of transcription and nucleotide excision repair within RNA polymerase II-transcribed genes of Saccharomyces cerevisiae.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed, 60 citations in OpenAlex.
- Transcription-coupled nucleotide excision repair: New insights revealed by genomic approaches.DNA repair · 2021Review
- Genome-wide role of Rad26 in promoting transcription-coupled nucleotide excision repair in yeast chromatin.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- DNA repair and recovery of RNA synthesis following exposure to ultraviolet light are delayed in long genes.Nucleic acids research · 2015Article
- Insights into how Spt5 functions in transcription elongation and repressing transcription coupled DNA repair.Nucleic acids research · 2014Article
- Rad26, the transcription-coupled repair factor in yeast, is required for removal of stalled RNA polymerase-II following UV irradiation.PloS one · 2013Article
- Tfb5 is partially dispensable for Rad26 mediated transcription coupled nucleotide excision repair in yeast.DNA repair · 2007Article
- Modulation of Rad26- and Rpb9-mediated DNA repair by different promoter elements.The Journal of biological chemistry · 2006Article
- Dissecting transcription-coupled and global genomic repair in the chromatin of yeast GAL1-10 genes.The Journal of biological chemistry · 2004Article
- Article
- Rpb4 and Rpb9 mediate subpathways of transcription-coupled DNA repair in Saccharomyces cerevisiae.The EMBO journal · 2002Article
- Transcription elongation factor Spt4 mediates loss of phosphorylated RNA polymerase II transcription in response to DNA damage.Nucleic acids research · 2002Article
- Defective nucleotide excision repair in yeast hpr1 and tho2 mutants.Nucleic acids research · 2002Article
- Spt4 modulates Rad26 requirement in transcription-coupled nucleotide excision repair.The EMBO journal · 2000Article
- Cell cycle-independent removal of UV-induced pyrimidine dimers from the promoter and the transcription initiation domain of the human CDC2 gene.Nucleic acids research · 2000Article
- Excision repair at the level of the nucleotide in the upstream control region, the coding sequence and in the region where transcription terminates of the Saccharomyces cerevisiae MFA2 gene and the role of RAD26.Nucleic acids research · 2000Article
- Article
- RNA polymerase II transcription suppresses nucleosomal modulation of UV-induced (6-4) photoproduct and cyclobutane pyrimidine dimer repair in yeast.Molecular and cellular biology · 1999Article
- Yeast RNA polymerase II transcription in vitro is inhibited in the presence of nucleotide excision repair: complementation of inhibition by Holo-TFIIH and requirement for RAD26.Molecular and cellular biology · 1998Article
- Saccharomyces cerevisiae mms19 mutants are deficient in transcription-coupled and global nucleotide excision repair.Nucleic acids research · 1997Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The molecular mechanism of transcription-coupled nucleotide excision repair in eukaryotes is poorly understood. The identification of the dual role of basal transcription factor TFIIH in DNA repair and transcription provided a plausible link between both processes. However, TFIIH is not part of the elongating transcription complex, suggesting that additional components are required to recruit TFIIH when RNA polymerase II (RNAPII) stalls at the site of DNA damage. Previously, we have shown that the yeast Rad26 protein is involved in transcription-coupled DNA repair. This paper describes the differential contribution of the Rad26 protein to efficient removal of UV-induced cyclobutane pyrimidine dimers (CPDs) from transcribed DNA. Two distinct regions within the transcribed strand of RNAPII-transcribed genes are identified that differ in their requirement for the RAD26 gene product. Using high-resolution repair analysis, we determined the in vivo repair kinetics of cyclobutane pyrimidine dimers positioned around the transcription initiation site of RNAPII-transcribed genes RPB2 and URA3. Although transcription-coupled repair is severely reduced in rad26 mutants, lesions positioned in a small region immediately downstream of transcription initiation are efficiently removed in the absence of Rad26. The observed transition in repair characteristics is abrupt and in excellent agreement with the region where TFIIH dissociates from RNAPII in vitro, strongly suggesting an inverse correlation between TFIIH association and Rad26 requirement. These data suggest that a transcription repair coupling factor (Rad26/CSB) is required for efficient repair only during the elongating stages of RNAPII transcription.
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