ArticleThe Journal of general physiology1997
Role of transmembrane segment S5 on gating of voltage-dependent K+ channels.
Article in The Journal of general physiology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
32 citing papers in PubMed, 66 citations in OpenAlex.
- Voltage gating by molecular subunits of Na+ and K+ ion channels: higher-dimensional cubic kinetics, rate constants, and temperature.Journal of neurophysiology · 2015Article
- Potassium channels in pancreatic duct epithelial cells: their role, function and pathophysiological relevance.Pflugers Archiv : European journal of physiology · 2015Review
- Modulation of Closed-State Inactivation in Kv2.1/Kv6.4 Heterotetramers as Mechanism for 4-AP Induced Potentiation.PloS one · 2015Article
- Deletion of the Kv2.1 delayed rectifier potassium channel leads to neuronal and behavioral hyperexcitability.Genes, brain, and behavior · 2014Article
- S3-S4 linker length modulates the relaxed state of a voltage-gated potassium channel.Biophysical journal · 2013Article
- The ladder-shaped polyether toxin gambierol anchors the gating machinery of Kv3.1 channels in the resting state.The Journal of general physiology · 2013Article
- Molecular determinants of U-type inactivation in Kv2.1 channels.Biophysical journal · 2011Article
- Uncoupling charge movement from channel opening in voltage-gated potassium channels by ruthenium complexes.The Journal of biological chemistry · 2011Article
- Mutations within the S4-S5 linker alter voltage sensor constraints in hERG K+ channels.Biophysical journal · 2010Article
- Functional effects of spinocerebellar ataxia type 13 mutations are conserved in zebrafish Kv3.3 channels.BMC neuroscience · 2010Article
- Sca13.Cerebellum (London, England) · 2008Article
- An activation gating switch in Kv1.2 is localized to a threonine residue in the S2-S3 linker.Biophysical journal · 2007Article
- Gating currents from a Kv3 subfamily potassium channel: charge movement and modification by BDS-II toxin.The Journal of physiology · 2007Article
- Ionic channel function in action potential generation: current perspective.Molecular neurobiology · 2007Review
- Models of the structure and voltage-gating mechanism of the shaker K+ channel.Biophysical journal · 2004Article
- Effects of Kv1.2 intracellular regions on activation of Kv2.1 channels.Biophysical journal · 2004Article
- Rate-limiting reactions determining different activation kinetics of Kv1.2 and Kv2.1 channels.The Journal of membrane biology · 2004Article
- Molecular coupling between voltage sensor and pore opening in the Arabidopsis inward rectifier K+ channel KAT1.The Journal of general physiology · 2003Article
- Kv3 potassium conductance is necessary and kinetically optimized for high-frequency action potential generation in hippocampal interneurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2003Article
- Antibodies and a cysteine-modifying reagent show correspondence of M current in neurons to KCNQ2 and KCNQ3 K+ channels.British journal of pharmacology · 2002Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The cytoplasmic half of S5 (5'S5) has been identified as part of the inner mouth of the pore based on evidence that mutations in this region greatly alter single channel conductance, 4-aminopyridine (4-AP) block and the rate of channel closing upon repolarization (deactivation). The latter effect, suggestive of a role for 5'S5 in channel gating was investigated in the present study. The biophysical properties of chimeric channels, in which the 5'S5 regions were exchanged between two host channels (Kv2.1 and Kv3.1) that differ in 4-AP sensitivity and deactivation rate, were examined in a Xenopus oocyte expression system. Exchange of 5'S5 between Kv2.1 and Kv3.1 confers steady-state voltage dependence of activation and rates of channel deactivation similar to those of the donor channel. The involvement of voltage-dependent gating was confirmed by the observation that exchanging the 5'S5 segment of Kv2.1 with that of Kv3.1 confers a change from slow to fast deactivation kinetics by accelerating the decay of off-gating charge movement. We suggest that a conformational change that extends from the voltage-sensor in S4 to the region of the pore lined by S5 regulates the stability of the open state. Therefore, the cytoplasmic end of S5, in addition to forming part of the conduction pathway near the inner mouth of the pore, also participates in the conformational rearrangements associated with late steps in channel activation and early steps in deactivation.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.