ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience1997
Microtubule transport from the cell body into the axons of growing neurons.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 56 citations in OpenAlex.
- Centrosome-dependent microtubule modifications set the conditions for axon formation.Cell reports · 2022Article
- Mini-review: Microtubule sliding in neurons.Neuroscience letters · 2021Review
- A Pictorial History of the Neuronal Cytoskeleton.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2021Article
- An in vivo brain-bacteria interface: the developing brain as a key regulator of innate immunity.NPJ Regenerative medicine · 2020Article
- Moonlighting Motors: Kinesin, Dynein, and Cell Polarity.Trends in cell biology · 2017Review
- Sliding of centrosome-unattached microtubules defines key features of neuronal phenotype.The Journal of cell biology · 2016Article
- A novel role for doublecortin and doublecortin-like kinase in regulating growth cone microtubules.Human molecular genetics · 2012Article
- Taxol-stabilized microtubules promote the formation of filaments from unmodified full-length Tau in vitro.Molecular biology of the cell · 2012Article
- Hooks and comets: The story of microtubule polarity orientation in the neuron.Developmental neurobiology · 2011Review
- Doublecortin associates with microtubules preferentially in regions of the axon displaying actin-rich protrusive structures.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2009Article
- Cytoskeletal requirements in axonal transport of slow component-b.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2008Article
- Hsp27 and axonal growth in adult sensory neurons in vitro.BMC neuroscience · 2005Article
- Role of actin filaments in the axonal transport of microtubules.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2004Article
- Rapid intermittent movement of axonal neurofilaments observed by fluorescence photobleaching.Molecular biology of the cell · 2001Article
- Neurofilaments are transported rapidly but intermittently in axons: implications for slow axonal transport.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2000Article
- Reorganization and movement of microtubules in axonal growth cones and developing interstitial branches.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1999Article
- Different contributions of microtubule dynamics and transport to the growth of axons and collateral sprouts.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1999Article
- An essential role for katanin in severing microtubules in the neuron.The Journal of cell biology · 1999Article
- Acute inactivation of tau has no effect on dynamics of microtubules in growing axons of cultured sympathetic neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1998Article
- Transport and turnover of microtubules in frog neurons depend on the pattern of axonal growth.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1998Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The present studies test the hypothesis that microtubules (MTs) are transported from the cell body into the axons of growing neurons. Dissociated sympathetic neurons were cultured using conditions that allow us to control the initiation of axon outgrowth. Neurons were injected with biotin-labeled tubulin (Bt-tub) and then stimulated to extend axons. The newly formed axons were then examined using immunofluorescence procedures for MTs with or without Bt-tub. Because the Bt-tub is fully assembly competent, all MTs that assemble after injection will contain Bt-tub. However, MTs that exist in the neuron at the time of injection and persist during the subsequent incubation will not contain Bt-tub. Because the neurons were injected before extending axons, MTs without Bt-tub are initially localized to the cell body. We specifically determined whether these MTs appeared in the newly formed axon. Such a result can only be explained by the transport of these MTs from their initial location in the cell body into the axon. The newly formed axons of many neurons contained MTs both with and without Bt-tub. MTs without Bt-tub were detected all along the axon and in some neurons represented a substantial portion of the total polymer in the proximal and middle regions of the axon. These results show that MTs are transported from the cell body into growing axons and that this transport is robust, delivering MTs to all regions of the newly formed axon.
Indexed as
Identifiers
9221779PMC6573214W2128997849What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.