ArticleThe Journal of experimental medicine1997
Human peripheral blood eosinophils express a functional c-kit receptor for stem cell factor that stimulates very late antigen 4 (VLA-4)-mediated cell adhesion to fibronectin and vascular cell adhesion molecule 1 (VCAM-1).
Article in The Journal of experimental medicine, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT01097694 (A 28 Week, Treatment Randomized, Double -Blind, Placebo-controlled Study of the Effects of cKit Inhibition by Imatinib in Patients With Severe Refractory Asthma), which is not on this map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
A 28 Week, Treatment Randomized, Double -Blind, Placebo-controlled Study of the Effects of cKit Inhibition by Imatinib in Patients With Severe Refractory Asthma (KIA)
Who cites it
25 citing papers in PubMed, 98 citations in OpenAlex.
- Meteorin-like protein/METRNL/Interleukin-41 ameliorates atopic dermatitis-like inflammation.Allergy · 2025Article
- Genetic Changes in Mastocytes and Their Significance in Mast Cell Tumor Prognosis and Treatment.Genes · 2024Review
- Stem cell factor inhibition reduces Th2 inflammation and cellular infiltration in a mouse model of eosinophilic esophagitis.Mucosal immunology · 2023Article
- KIT as a master regulator of the mast cell lineage.The Journal of allergy and clinical immunology · 2022Review
- C-KIT Expression in Orbital Cavernous Venous Hemangiomas.Biomolecules · 2021Article
- Human eosinophils and mast cells: Birds of a feather flock together.Immunological reviews · 2018Review
- Association of stem cell factor gene expression with severity and atopic state in patients with bronchial asthma.Respiratory research · 2017Article
- Bidirectional Mast Cell-Eosinophil Interactions in Inflammatory Disorders and Cancer.Frontiers in medicine · 2017Review
- Differential expression and biochemical activity of the immune receptor Tim-3 in healthy and malignant human myeloid cells.Oncotarget · 2015Article
- Platelet-driven leukotriene C4-mediated airway inflammation in mice is aspirin-sensitive and depends on T prostanoid receptors.Journal of immunology (Baltimore, Md. : 1950) · 2015Article
- Functional deregulation of KIT: link to mast cell proliferative diseases and other neoplasms.Immunology and allergy clinics of North America · 2014Review
- Intranasal sirna targeting c-kit reduces airway inflammation in experimental allergic asthma.International journal of clinical and experimental pathology · 2014Article
- Effects of imatinib mesylate on pulmonary allergic vasculitis in a murine model.International journal of rheumatic diseases · 2013Article
- Eosinophils in innate immunity: an evolving story.Cell and tissue research · 2011Review
- Endothelial-mesenchymal transition in bleomycin-induced pulmonary fibrosis.American journal of respiratory cell and molecular biology · 2010Article
- Hypoxia modulates human eosinophil function.Clinical and molecular allergy : CMA · 2010Article
- FIP1L1/PDGFR alpha-associated systemic mastocytosis.International archives of allergy and immunology · 2010Review
- Expression of ADAMs ("a disintegrin and metalloprotease") in the human lung.Virchows Archiv : an international journal of pathology · 2009Article
- Pharmacological targeting of the KIT growth factor receptor: a therapeutic consideration for mast cell disorders.British journal of pharmacology · 2008Review
- Eosinophil activation of fibroblasts from chronic allergen-induced disease utilizes stem cell factor for phenotypic changes.The American journal of pathology · 2008Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
We evaluated mature peripheral blood eosinophils for their expression of the surface tyrosine kinase, c-kit, the receptor for the stromal cell-derived cytokine, stem cell factor (SCF). Cytofluorographic analysis revealed that c-kit was expressed on the purified peripheral blood eosinophils from 8 of 8 donors (4 nonatopic and 4 atopic) (mean channel fluorescence intensity 2.0- 3. 6-fold, average 2.8 +/- 0.6-fold, greater than the negative control). The uniform and selective expression of c-kit by eosinophils was confirmed by immunohistochemical analysis of peripheral blood buffy coats. The functional integrity of c-kit was demonstrated by the capacity of 100 ng/ml (5 nM) of recombinant human (rh) SCF to increase eosinophil adhesion to 3, 10, and 30 microg/ml of immobilized FN40, a 40-kD chymotryptic fragment of plasma fibronectin, in 15 min by 7.7 +/- 1.4-, 5.3 +/- 3.3-, and 5.4 +/- 0. 2-fold, respectively, and their adhesion to 0.1, 0.5, and 1.0 microg/ml vascular cell adhesion molecule-1 (VCAM-1), by 12.7 +/- 9. 2-, 3.8 +/- 2.5-, and 1.7 +/- 0.6-fold, respectively. The SCF-stimulated adhesion occurred without concomitant changes in surface integrin expression, thereby indicating an avidity-based mechanism. rhSCF (100 ng/ml, 5 nM) was comparable to rh eotaxin (200 ng/ml, 24 nM) in stimulating adhesion. Cell adhesion to FN40 was completely inhibited with antibodies against the alpha4 and beta1 integrin subunits, revealing that the SCF/c-kit adhesion effect was mediated by a single integrin heterodimer, very late antigen 4 (VLA-4). Thus, SCF represents a newly recognized stromal ligand for the activation of eosinophils for VLA-4-mediated adhesion, which could contribute to the exit of these cells from the blood, their tissue localization, and their prominence in inflammatory lesions.
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