ArticleMolecular biotechnology1997
Decontamination of polymerase chain reaction reagents for detection of low concentrations of 16S rRNA genes.
Article in Molecular biotechnology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 53 citations in OpenAlex.
- Recombinant Thermostable DNA Polymerases: Current Approaches to Production, Molecular Engineering, and Applications in Biotechnology and Diagnostics.International journal of molecular sciences · 2026Review
- Sample Collection Methods in Upper Gastrointestinal Research.Journal of Korean medical science · 2023Review
- High-Level Production of DNA-Specific Endonuclease AsEndI with Synonymous Codon and its Potential Utilization for Removing DNA Contamination.Applied biochemistry and biotechnology · 2018Article
- Occurrence of Fungal DNA Contamination in PCR Reagents: Approaches to Control and Decontamination.Journal of clinical microbiology · 2016Article
- Reagent and laboratory contamination can critically impact sequence-based microbiome analyses.BMC biology · 2014Article
- An efficient multistrategy DNA decontamination procedure of PCR reagents for hypersensitive PCR applications.PloS one · 2010Article
- Optimizing Taq polymerase concentration for improved signal-to-noise in the broad range detection of low abundance bacteria.PloS one · 2009Article
- 16S-Ribosomal DNA to diagnose culture-negative endocarditis.Current infectious disease reports · 2008Article
- An improved method of elimination of DNA from PCR reagents.Molecular diagnosis : a journal devoted to the understanding of human disease through the clinical application of molecular biology · 2005Article
- Culture-negative neonatal meningitis and endocarditis caused by Streptococcus agalactiae.Journal of clinical microbiology · 2004Article
- Presence of bacterial phage-like DNA sequences in commercial Taq DNA polymerase reagents.Journal of clinical microbiology · 2004Article
- Prospective study of use of PCR amplification and sequencing of 16S ribosomal DNA from cerebrospinal fluid for diagnosis of bacterial meningitis in a clinical setting.Journal of clinical microbiology · 2004Article
- Optimization of real-time PCR assay for rapid and sensitive detection of eubacterial 16S ribosomal DNA in platelet concentrates.Journal of clinical microbiology · 2003Article
- DNase pretreatment of master mix reagents improves the validity of universal 16S rRNA gene PCR results.Journal of clinical microbiology · 2003Article
- Comparison of different decontamination methods for reagents to detect low concentrations of bacterial 16S DNA by real-time-PCR.Molecular biotechnology · 2002Article
- Comparison of 16S rRNA gene PCR and BACTEC 9240 for detection of neonatal bacteremia.Journal of clinical microbiology · 2000Article
- Contamination and sensitivity issues with a real-time universal 16S rRNA PCR.Journal of clinical microbiology · 2000Article
- A broad-range PCR technique for the diagnosis of infective endocarditis.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We describe a polymerase chain reaction (PCR) that allowed detection of rRNA consensus sequences from the DNA extracted from a wide range of bacterial species in amounts as low as 10 fg. To avoid false positive results with universal primers for 16S rRNA PCR, contaminating DNA had to be eliminated from the polymerase preparations. Decontamination was undertaken before PCR to optimize treatment with DNaseI, and was followed by DNase inactivation at 94 degrees C for 50 min, which eliminated contaminating DNA at concentrations of up to 100 pg. After optimization of PCR conditions for each polymerase. Deep Vent Exo-polymerase (New England Biolabs, Beverly, MA), and super-Taq polymerase (HT Biotechnology, Cambridge, UK) were more effective than Ampli-Taq polymerase (Perkin-Elmer Cetus, Norwalk CT), Ampli-Taq LD polymerase (Perkin-Elmer Cetus) or Deep-vent polymerase (New England Biolabs). The technique described in this article might prove to be a universal method for PCR detection of small numbers of unidentified bacteria in usually sterile clinical sites, such as blood and cerebrospinal fluids, in which a broad spectrum of pathogens can be expected.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.