Evidence map›Paper›PMID 9219230›Full record

ArticleMolecular biotechnology1997

The use of RNA probes for the analysis of gene expression.

D Belin

Abstract read
PubMed Publisher
In one paragraph

Article in Molecular biotechnology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. An overview of structural approaches to study therapeutic RNAs.Frontiers in molecular biosciences · 2022
    Review
  2. Article
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

1 author.

D BelinDepartment of Pathology, University of Geneva Medical School, CMU, Switzerland. dominique.belin@medecine.unigo.ch

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The monomeric bacteriophage RNA polymerases allow the synthesis of virtually any RNA molecule in unlimited quantity. In this protocol, we describe the preparation of plasmid and PCR-derived templates. A basic transcription protocol is provided with several optional modifications. The use of RNA probes in Northern blot hybridization and in RNase protection assays is described. The relative advantages and pitfalls of these two methods to quantitatively detect mRNA targets are discussed.

Indexed as

Gene ExpressionRNA ProbesAnimalsBlotting, NorthernNucleic Acid HybridizationPlasmidsPolymerase Chain ReactionRibonucleasesTemplates, GeneticRibonucleasesRNA Probes

Identifiers

PMID9219230

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.