ArticleThe EMBO journal1997
Modulation of murine melanocyte function in vitro by agouti signal protein.
Article in The EMBO journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 1 of them a synthesis that pooled it.
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Who cites it
25 citing papers in PubMed, 1 synthesis or guideline pooled it, 96 citations in OpenAlex.
- Topical treatment strategies to manipulate human skin pigmentation.Advanced drug delivery reviews · 2020Pooled it
- Transcriptomic and Metabolomic Analyses Reveal Molecular Regulatory Networks for Pigmentation Deposition in Sheep.International journal of molecular sciences · 2024Article
- Pharmacological Modulation of Melanocortin 1 Receptor Signaling by Mrap Proteins inFrontiers in endocrinology · 2022Article
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- Measurement of Melanin Metabolism in Live Cells by [U-The Journal of investigative dermatology · 2021Article
- Participation of keratinocyte- and fibroblast-derived factors in melanocyte homeostasis, the response to UV, and pigmentary disorders.Pigment cell & melanoma research · 2021Review
- Development of hMC1R Selective Small Agonists for Sunless Tanning and Prevention of Genotoxicity of UV in Melanocytes.The Journal of investigative dermatology · 2021Article
- A quantitative trait locus on chromosome 2 was identified that accounts for a substantial proportion of phenotypic variance of the yellow plumage color in chicken.Poultry science · 2020Article
- Morphological Characteristics and Comparative Transcriptome Analysis of Three Different Phenotypes ofFrontiers in genetics · 2019Article
- MC1R: Front and Center in the Bright Side of Dark Eumelanin and DNA Repair.International journal of molecular sciences · 2018Review
- Comparative transcriptome analysis of molecular mechanism underlying gray-to-red body color formation in red crucian carp (Carassius auratus, red var.).Fish physiology and biochemistry · 2017Article
- Melanocortin 1 Receptor: Structure, Function, and Regulation.Frontiers in genetics · 2016Review
- MC1R, the cAMP pathway, and the response to solar UV: extending the horizon beyond pigmentation.Pigment cell & melanoma research · 2014Review
- Comparative transcriptome analysis reveals the genetic basis of skin color variation in common carp.PloS one · 2014Article
- Melanocortin 1 receptor regulates melanoma cell migration by controlling syndecan-2 expression.The Journal of biological chemistry · 2012Article
- Targeting protein-trafficking pathways alters melanoma treatment sensitivity.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Involvement of the melanocortin-1 receptor in acute pain and pain of inflammatory but not neuropathic origin.PloS one · 2010Article
- Agouti protein, mahogunin, and attractin in pheomelanogenesis and melanoblast-like alteration of melanocytes: a cAMP-independent pathway.Pigment cell & melanoma research · 2009Article
- Microarray analysis sheds light on the dedifferentiating role of agouti signal protein in murine melanocytes via the Mc1r.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- MHC class II presentation of gp100 epitopes in melanoma cells requires the function of conventional endosomes and is influenced by melanosomes.Journal of immunology (Baltimore, Md. : 1950) · 2008Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
Molecular and biochemical mechanisms that switch melanocytes between the production of eumelanin or pheomelanin involve the opposing action of two intercellular signaling molecules, alpha-melanocyte-stimulating hormone (MSH) and agouti signal protein (ASP). In this study, we have characterized the physiological effects of ASP on eumelanogenic melanocytes in culture. Following exposure of black melan-a murine melanocytes to purified recombinant ASP in vitro, pigmentation was markedly inhibited and the production of eumelanosomes was decreased significantly. Melanosomes that were produced became pheomelanosome-like in structure, and chemical analysis showed that eumelanin production was significantly decreased. Melanocytes treated with ASP also exhibited time- and dose-dependent decreases in melanogenic gene expression, including those encoding tyrosinase and tyrosinase-related proteins 1 and 2. Conversely, melanocytes exposed to MSH exhibited an increase in tyrosinase gene expression and function. Simultaneous addition of ASP and MSH at approximately equimolar concentrations produced responses similar to those elicited by the hormone alone. These results demonstrate that eumelanogenic melanocytes can be induced in culture by ASP to exhibit features characteristic of pheomelanogenesis in vivo. Our data are consistent with the hypothesis that the effects of ASP on melanocytes are not mediated solely by inhibition of MSH binding to its receptor, and provide a cell culture model to identify novel factors whose presence is required for pheomelanogenesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.