ArticleThe EMBO journal1997
Lipid remodeling leads to the introduction and exchange of defined ceramides on GPI proteins in the ER and Golgi of Saccharomyces cerevisiae.
Article in The EMBO journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 118 citations in OpenAlex.
- Vps13-like proteins provide phosphatidylethanolamine for GPI anchor synthesis in the ER.The Journal of cell biology · 2022Article
- Deficiency of GPI Glycan Modification by Ethanolamine Phosphate Results in Increased Adhesion and Immune Resistance ofFrontiers in cellular and infection microbiology · 2021Article
- Aspergillus fumigatus phosphoethanolamine transferase gene gpi7 is required for proper transportation of the cell wall GPI-anchored proteins and polarized growth.Scientific reports · 2019Article
- Probing de novo sphingolipid metabolism in mammalian cells utilizing mass spectrometry.Journal of lipid research · 2018Article
- Limited ER quality control for GPI-anchored proteins.The Journal of cell biology · 2016Article
- Biosynthesis of GPI-anchored proteins: special emphasis on GPI lipid remodeling.Journal of lipid research · 2016Review
- Dual functions of the trans-2-enoyl-CoA reductase TER in the sphingosine 1-phosphate metabolic pathway and in fatty acid elongation.The Journal of biological chemistry · 2014Article
- A novel Sit4 phosphatase complex is involved in the response to ceramide stress in yeast.Oxidative medicine and cellular longevity · 2013Article
- Total synthesis of a glycosylphosphatidylinositol anchor of the human lymphocyte CD52 antigen.Chemistry (Weinheim an der Bergstrasse, Germany) · 2012Article
- Chemical synthesis of glycosylphosphatidylinositol anchors.Advances in carbohydrate chemistry and biochemistry · 2012Review
- Distribution and functions of sterols and sphingolipids.Cold Spring Harbor perspectives in biology · 2011Review
- Yeast cells lacking all known ceramide synthases continue to make complex sphingolipids and to incorporate ceramides into glycosylphosphatidylinositol (GPI) anchors.The Journal of biological chemistry · 2011Article
- The Sphingolipid Biosynthetic Pathway Is a Potential Target for Chemotherapy against Chagas Disease.Enzyme research · 2011Article
- Incorporation of ceramides into Saccharomyces cerevisiae glycosylphosphatidylinositol-anchored proteins can be monitored in vitro.Eukaryotic cell · 2009Article
- Yeast ARV1 is required for efficient delivery of an early GPI intermediate to the first mannosyltransferase during GPI assembly and controls lipid flow from the endoplasmic reticulum.Molecular biology of the cell · 2008Article
- Saccharomyces cerevisiae CWH43 is involved in the remodeling of the lipid moiety of GPI anchors to ceramides.Molecular biology of the cell · 2007Article
- Yeast sphingolipids do not need to contain very long chain fatty acids.The Biochemical journal · 2007Article
- PER1 is required for GPI-phospholipase A2 activity and involved in lipid remodeling of GPI-anchored proteins.Molecular biology of the cell · 2006Article
- PGAP2 is essential for correct processing and stable expression of GPI-anchored proteins.Molecular biology of the cell · 2006Article
- Characterization of the inositol phosphorylceramide synthase activity from Trypanosoma cruzi.The Biochemical journal · 2005Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Previous experiments with Saccharomyces cerevisiae had suggested that diacylglycerol-containing glycosylphosphatidylinositols (GPIs) are added to newly synthesized proteins in the endoplasmic reticulum (ER) and that ceramides subsequently are incorporated into GPI proteins by lipid remodeling. Here we prove this hypothesis by labeling yeast cells with [3H]dihydrosphingosine ([3H]DHS) and showing that this tracer is incorporated into many GPI proteins even when protein synthesis and, hence, anchor addition, is blocked by cycloheximide. [3H]DHS incorporation is greatly enhanced if endogenous synthesis of DHS is inhibited by myriocin. Labeled GPI anchors contain three types of ceramides which, based on previous and present results, are identified as DHS-C26:0, phytosphingosine-C26:0 and phytosphingosine-C26:0-OH, the latter being found only on proteins which have reached the Golgi. Lipid remodeling can occur both in the ER and in a later secretory compartment. In addition, ceramide is incorporated into GPI proteins a long time after their initial synthesis by a process in which one ceramide gets replaced by another ceramide. Remodeling outside the ER requires vesicular flow from the ER to the Golgi, possibly to supply the remodeling enzymes with ceramides.
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