ArticleThe EMBO journal1997
Alternative lipid remodelling pathways for glycosylphosphatidylinositol membrane anchors in Saccharomyces cerevisiae.
Article in The EMBO journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 89 citations in OpenAlex.
- Review
- The Glycosylphosphatidylinositol Anchor: A Linchpin for Cell Surface Versatility of Trypanosomatids.Frontiers in cell and developmental biology · 2021Review
- MoPer1 is required for growth, conidiogenesis, and pathogenicity in Magnaporthe oryzae.Rice (New York, N.Y.) · 2018Article
- Yeast Gup1(2) Proteins Are Homologues of the Hedgehog Morphogens Acyltransferases HHAT(L): Facts and Implications.Journal of developmental biology · 2016Review
- Biosynthesis of GPI-anchored proteins: special emphasis on GPI lipid remodeling.Journal of lipid research · 2016Review
- The Sphingolipid Biosynthetic Pathway Is a Potential Target for Chemotherapy against Chagas Disease.Enzyme research · 2011Article
- Incorporation of ceramides into Saccharomyces cerevisiae glycosylphosphatidylinositol-anchored proteins can be monitored in vitro.Eukaryotic cell · 2009Article
- Yeast ARV1 is required for efficient delivery of an early GPI intermediate to the first mannosyltransferase during GPI assembly and controls lipid flow from the endoplasmic reticulum.Molecular biology of the cell · 2008Article
- Saccharomyces cerevisiae CWH43 is involved in the remodeling of the lipid moiety of GPI anchors to ceramides.Molecular biology of the cell · 2007Article
- Fatty acid remodeling of GPI-anchored proteins is required for their raft association.Molecular biology of the cell · 2007Article
- Yeast sphingolipids do not need to contain very long chain fatty acids.The Biochemical journal · 2007Article
- PER1 is required for GPI-phospholipase A2 activity and involved in lipid remodeling of GPI-anchored proteins.Molecular biology of the cell · 2006Article
- GUP1 of Saccharomyces cerevisiae encodes an O-acyltransferase involved in remodeling of the GPI anchor.Molecular biology of the cell · 2006Article
- PGAP2 is essential for correct processing and stable expression of GPI-anchored proteins.Molecular biology of the cell · 2006Article
- Characterization of the inositol phosphorylceramide synthase activity from Trypanosoma cruzi.The Biochemical journal · 2005Article
- Identification and biophysical characterization of a very-long-chain-fatty-acid-substituted phosphatidylinositol in yeast subcellular membranes.The Biochemical journal · 2004Article
- An introduction to plant sphingolipids and a review of recent advances in understanding their metabolism and function.The New phytologist · 2004Review
- Formation and remodeling of inositolphosphoceramide during differentiation of Trypanosoma cruzi from trypomastigote to amastigote.Eukaryotic cell · 2003Article
- A specific structural requirement for ergosterol in long-chain fatty acid synthesis mutants important for maintaining raft domains in yeast.Molecular biology of the cell · 2002Article
- The GPI transamidase complex of Saccharomyces cerevisiae contains Gaa1p, Gpi8p, and Gpi16p.Molecular biology of the cell · 2001Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Glycosylphosphatidylinositol (GPI)-anchored membrane proteins of Saccharomyces cerevisiae exist with two types of lipid moiety--diacylglycerol or ceramide--both of which contain 26:0 fatty acids. To understand at which stage of biosynthesis these long-chain fatty acids become incorporated into diacylglycerol anchors, we compared the phosphatidylinositol moieties isolated from myo-[2-(3)H]inositol-labelled protein anchors and from GPI intermediates. There is no evidence for the presence of long-chain fatty acids in any intermediate of GPI biosynthesis. However, GPI-anchored proteins contain either the phosphatidylinositol moiety characteristic of the precursor lipids or a version with a long-chain fatty acid in the sn-2 position of glycerol. The introduction of long-chain fatty acids into sn-2 occurs in the endoplasmic reticulum (ER) and is independent of the sn-2-specific acyltransferase SLC1. Analysis of ceramide anchors revealed the presence of two types of ceramide, one added in the ER and another more polar molecule which is found only on proteins which have reached the mid Golgi. In summary, the lipid of GPI-anchored proteins can be exchanged by at least three different remodelling pathways: (i) remodelling from diacylglycerol to ceramide in the ER as proposed previously; (ii) remodelling from diacylglycerol to a more hydrophobic diacylglycerol with a long-chain fatty acid in sn-2 in the ER; and (iii) remodelling to a more polar ceramide in the Golgi.
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