ArticleThe EMBO journal1997
Targeting and translocation of proteins into the hydrogenosome of the protist Trichomonas: similarities with mitochondrial protein import.
Article in The EMBO journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
43 citing papers in PubMed, 126 citations in OpenAlex.
- A hybrid TIM complex mediates protein import into hydrogenosomes of Trichomonas vaginalis.BMC biology · 2024Article
- Down the membrane hole: Ion channels in protozoan parasites.PLoS pathogens · 2022Review
- Proteomic Analysis of Trichomonas vaginalis Phagolysosome, Lysosomal Targeting, and Unconventional Secretion of Cysteine Peptidases.Molecular & cellular proteomics : MCP · 2022Article
- N-Terminal Segment ofBiomolecules · 2020Article
- Endomembrane Protein Trafficking Regulated by a TvCyP2 Cyclophilin in the Protozoan Parasite, Trichomonas vaginalis.Scientific reports · 2020Article
- Hydrogenosomal tail-anchored proteins are targeted to both mitochondria and ER upon their expression in yeast cells.PloS one · 2020Article
- Mitochondrial dynamics in parasitic protists.PLoS pathogens · 2019Review
- Article
- Triplet-pore structure of a highly divergent TOM complex of hydrogenosomes in Trichomonas vaginalis.PLoS biology · 2019Article
- Mitochondrial Glycolysis in a Major Lineage of Eukaryotes.Genome biology and evolution · 2018Article
- N-Terminal Presequence-Independent Import of Phosphofructokinase into Hydrogenosomes of Trichomonas vaginalis.Eukaryotic cell · 2015Article
- Novel functions of an iron-sulfur flavoprotein from Trichomonas vaginalis hydrogenosomes.Antimicrobial agents and chemotherapy · 2014Article
- Trypanosome alternative oxidase possesses both an N-terminal and internal mitochondrial targeting signal.Eukaryotic cell · 2014Article
- Analysis of the Sam50 translocase of excavate organisms supports evolution of divergent organelles from a common endosymbiotic event.Bioscience reports · 2013Article
- A machine learning approach to identify hydrogenosomal proteins in Trichomonas vaginalis.Eukaryotic cell · 2012Article
- The Trichomonas vaginalis hydrogenosome proteome is highly reduced relative to mitochondria, yet complex compared with mitosomes.International journal for parasitology · 2011Article
- The core components of organelle biogenesis and membrane transport in the hydrogenosomes of Trichomonas vaginalis.PloS one · 2011Article
- Mitochondrion-related organelles in eukaryotic protists.Annual review of microbiology · 2010Review
- The protein import channel in the outer mitosomal membrane of Giardia intestinalis.Molecular biology and evolution · 2009Article
- Giardia, Entamoeba, and Trichomonas enzymes activate metronidazole (nitroreductases) and inactivate metronidazole (nitroimidazole reductases).Antimicrobial agents and chemotherapy · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Trichomonads are early-diverging eukaryotes that lack both mitochondria and peroxisomes. They do contain a double membrane-bound organelle, called the hydrogenosome, that metabolizes pyruvate and produces ATP. To address the origin and biological nature of hydrogenosomes, we have established an in vitro protein import assay. Using purified hydrogenosomes and radiolabeled hydrogenosomal precursor ferredoxin (pFd), we demonstrate that protein import requires intact organelles, ATP and N-ethylmaleimide-sensitive cytosolic factors. Protein import is also affected by high concentrations of the protonophore, m-chlorophenylhydrazone (CCCP). Binding and translocation of pFd into hydrogenosomes requires the presence of an eight amino acid N-terminal presequence that is similar to presequences found on all examined hydrogenosomal proteins. Upon import, pFd is processed to a size consistent with cleavage of the presequence. Mutation of a conserved leucine at position 2 in the presequence to a glycine disrupts import of pFd into the organelle. Interestingly, a comparison of hydrogenosomal and mitochondrial protein presequences reveals striking similarities. These data indicate that mechanisms underlying protein targeting and biogenesis of hydrogenosomes and mitochondria are similar, consistent with the notion that these two organelles arose from a common endosymbiont.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.