ArticleThe EMBO journal1997
Crystal structure of human glyoxalase I--evidence for gene duplication and 3D domain swapping.
Article in The EMBO journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers.
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Who cites it
65 citing papers in PubMed, 235 citations in OpenAlex.
- Gut microbial-host isozymes: A novel perspective on gut microbiota-host interactions.iScience · 2026Review
- Discovery of hydroxytriazole as a potential glyoxalase-I inhibitor utilizing computer-aided drug design techniques.Scientific reports · 2026Article
- Structure-Based Discovery of Glyoxalase-I Inhibitors: Evaluating the Role of Active Site Water Molecules.Drug design, development and therapy · 2026Article
- Genome-Wide Analysis and Expression Profiling of Glyoxalase Gene Families Under Abiotic Stresses in Cucumber (International journal of molecular sciences · 2024Article
- Novel anthraquinone amide derivatives as potential glyoxalase-I inhibitors.Journal of medicine and life · 2024Article
- Rapid transcriptomic and physiological changes in the freshwater pennate diatom Mayamaea pseudoterrestris in response to copper exposure.DNA research : an international journal for rapid publication of reports on genes and genomes · 2022Article
- Salicylate metal-binding isosteres as fragments for metalloenzyme inhibition.Chemical science · 2022Article
- Role of the Glyoxalase System in Breast Cancer and Gynecological Cancer-Implications for Therapeutic Intervention: a Review.Frontiers in oncology · 2022Review
- Genome-Wide Expression Analysis of Glyoxalase I Genes Under Hyperosmotic Stress and Existence of a Stress-Responsive Mitochondrial Glyoxalase I Activity in Durum Wheat (Frontiers in plant science · 2022Article
- Glyoxalase System in the Progression of Skin Aging and Skin Malignancies.International journal of molecular sciences · 2020Review
- Interplay among Oxidative Stress, Methylglyoxal Pathway and S-Glutathionylation.Antioxidants (Basel, Switzerland) · 2020Review
- Salt-Tolerant Phenomena, Sequencing and Characterization of aPlants (Basel, Switzerland) · 2020Article
- Aromatization of natural products by a specialized detoxification enzyme.Nature chemical biology · 2020Article
- Expression, purification, characterization and in silico analysis of newly isolated hydrocarbon degrading bleomycin resistance dioxygenase.Molecular biology reports · 2020Article
- Reconstructing the Remote Origins of a Fold Singleton from a Flavodoxin-Like Ancestor.Biochemistry · 2019Article
- Discovery of a nanomolar inhibitor of the human glyoxalase-I enzyme using structure-based poly-pharmacophore modelling and molecular docking.Journal of computer-aided molecular design · 2019Article
- Structural basis of dimerization and dual W-box DNA recognition by rice WRKY domain.Nucleic acids research · 2019Article
- Targeting Metalloenzymes for Therapeutic Intervention.Chemical reviews · 2019Review
- Resistance to Enediyne Antitumor Antibiotics by Sequestration.Cell chemical biology · 2018Article
- Dissecting the Physiological Function of Plant Glyoxalase I and Glyoxalase I-Like Proteins.Frontiers in plant science · 2018Article
5 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The zinc metalloenzyme glyoxalase I catalyses the glutathione-dependent inactivation of toxic methylglyoxal. The structure of the dimeric human enzyme in complex with S-benzyl-glutathione has been determined by multiple isomorphous replacement (MIR) and refined at 2.2 A resolution. Each monomer consists of two domains. Despite only low sequence homology between them, these domains are structurally equivalent and appear to have arisen by a gene duplication. On the other hand, there is no structural homology to the 'glutathione binding domain' found in other glutathione-linked proteins. 3D domain swapping of the N- and C-terminal domains has resulted in the active site being situated in the dimer interface, with the inhibitor and essential zinc ion interacting with side chains from both subunits. Two structurally equivalent residues from each domain contribute to a square pyramidal coordination of the zinc ion, rarely seen in zinc enzymes. Comparison of glyoxalase I with other known structures shows the enzyme to belong to a new structural family which includes the Fe2+-dependent dihydroxybiphenyl dioxygenase and the bleomycin resistance protein. This structural family appears to allow members to form with or without domain swapping.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.