ArticleThe Journal of physiology1997
In vivo downregulation of M2 receptors revealed by measurement of muscarinic K+ current in cultured guinea-pig atrial myocytes.
Article in The Journal of physiology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 21 citations in OpenAlex.
- Autocrine signaling via A(1) adenosine receptors causes downregulation of M(2) receptors in adult rat atrial myocytes in vitro.Pflugers Archiv : European journal of physiology · 2011Article
- Expression of cTnI-R145G affects shortening properties of adult rat cardiomyocytes.Pflugers Archiv : European journal of physiology · 2008Article
- Generation of a constitutive Na+-dependent inward-rectifier current in rat adult atrial myocytes by overexpression of Kir3.4.The Journal of physiology · 2007Article
- Dynamics of receptor/G protein coupling in living cells.The EMBO journal · 2005Article
- Voltage dependence of ATP-dependent K+ current in rat cardiac myocytes is affected by IK1 and IK(ACh).The Journal of physiology · 2004Article
- New roles for RGS2, 5 and 8 on the ratio-dependent modulation of recombinant GIRK channels expressed in Xenopus oocytes.The Journal of physiology · 1999Article
- G-protein coupled receptor kinases as modulators of G-protein signalling.The Journal of physiology · 1999Review
- Non-receptor-mediated activation of IK(ATP) and inhibition of IK(ACh) by diadenosine polyphosphates in guinea-pig atrial myocytes.The Journal of physiology · 1998Article
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Authors and funding
3 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
1. Muscarinic K+ current (IK(ACh)) elicited by acetylcholine (ACh) was measured in guinea-pig atrial myocytes, which were either freshly isolated or cultured for up to 8 days. 2. The half-time of activation of inward IK(ACh) by a saturating concentration (10 microM) of ACh decreased from approximately 400 ms (in freshly isolated cells) to 250 ms after 6 days in culture. This was paralleled by an increase in the fast desensitizing component of IK(ACh). The density of steady-state currents was not changed. Downregulation of M2 receptors by long-term treatment of isolated myocytes with carbachol in vitro had opposite effects. 3. The EC50 of ACh for the activation of steady-state IK(ACh) was reduced from 5 x 10(-7) M (day 0) to 8 x 10(-8) M (day 6). The shift in EC50 occurred with a half-time of about 2 days, similar to the recovery from downregulation induced by treating atrial myocytes with carbachol in vitro. 4. The increase in sensitivity to ACh can be accounted for by an approximately 6-fold increase in the density of M2 receptors. 5. It is concluded that sensitization in culture reflects recovery from downregulation of M2 receptors due to the tonic vagal input to the heart in the intact animal.
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