ArticleJournal of clinical pathology1997
Tissue preparation for immunocytochemistry.
Article in Journal of clinical pathology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 107 citations in OpenAlex.
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- Incidence of NUT carcinoma in Western Australia from 1989 to 2014: a review of pediatric and adolescent cases from Perth Children's Hospital.BMC cancer · 2021Review
- Impact of Preanalytical Factors on the Measurement of Tumor Tissue Biomarkers Using Immunohistochemistry.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2021Article
- Imaging-Based Reporter Systems to Define CVB-Induced Membrane Remodeling in Living Cells.Viruses · 2020Article
- Comparison of Fixation Methods for Preservation Cytology Specimens of Cell Block Preparation Using 10% Neutral Buffer Formalin and 96% Alcohol Fixation in E-cadherin and Ki-67 Immunohistochemical Examination.Open access Macedonian journal of medical sciences · 2019Article
- Prognostic and Predictive Immunohistochemistry-Based Biomarkers in Cancer and Immunotherapy.Hematology/oncology clinics of North America · 2019Review
- Blockage of SLC31A1-dependent copper absorption increases pancreatic cancer cell autophagy to resist cell death.Cell proliferation · 2019Article
- Understanding preanalytical variables and their effects on clinical biomarkers of oncology and immunotherapy.Seminars in cancer biology · 2018Review
- Re-Appraisal of Estrogen Receptor Negative/Progesterone Receptor Positive (ER-/PR+) Breast Cancer Phenotype: True Subtype or Technical Artefact?Pathology oncology research : POR · 2018Article
- Cytological Smear and Cell Block Versus Tissue Biopsies in the Diagnosis of Malignant Tumours in Non-Gynaecologic Specimens.Ethiopian journal of health sciences · 2018Article
- The penetration of methanol into bovine cardiac and hepatic tissues is faster than ethanol and formalin.European journal of histochemistry : EJH · 2018Article
- Histological validation of diffusion MRI fiber orientation distributions and dispersion.NeuroImage · 2018Article
- Epitope Preservation Methods for Tissue Microarrays: Longitudinal Prospective Study.American journal of clinical pathology · 2017Article
- Comparison of 3D orientation distribution functions measured with confocal microscopy and diffusion MRI.NeuroImage · 2016Article
- Obstacles to precision oncology: confronting current factors affecting the successful introduction of biomarkers to the clinic.Cellular oncology (Dordrecht, Netherlands) · 2015Review
- Garbage in, garbage out: a critical evaluation of strategies used for validation of immunohistochemical biomarkers.Molecular oncology · 2014Review
- Study assessing the quality of quantification of estrogen receptor protein expression by immunohistochemistry and gene expression in breast cancer.Pathology research international · 2014Article
- Immunohistochemistry of pancreatic neoplasia.Methods in molecular biology (Clifton, N.J.) · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimsTo investigate the effect of tissue preparation on immunostaining and to establish whether there is a standard tissue preparation schedule that allows optimal demonstration of all antigens.
methodsBlocks of tonsil were subjected to variations to a standard fixation, processing, and section preparation schedule. The sections were stained with five antibodies-L26 (CD20), UCHL1 (CD45RO), CD3, vimentin, and anti-kappa light chain--using the streptavidinbiotin immunostaining technique. When further investigation was necessary, other tissues and antibodies were used and where weak immunostaining was obtained the use of microwave pretreatment to improve staining was tested.
resultsSeveral factors involved in fixation were found to affect immunoreactivity. These included the duration, pH, and type of fixative used. In tissue processing only temperature and the duration of the dehydration and wax infiltration steps affected immunoreactivity. Of all the factors investigated, the temperature and duration of the section drying had the greatest effect. In contrast, long term storage of cut sections before immunostaining had no effect on the reactivity of the antibodies tested. Antibodies were found to be affected by alterations to tissue preparation by varying degrees, UCHL1 and vimentin being the most susceptible to changes in fixation and L26 to changes in processing. Where weak staining occurred, microwave pretreatment was generally found to eliminate the problem.
conclusionsThere is no standard tissue preparation schedule for the optimal demonstration of all antigens. Factors involved in all aspects of tissue preparation can affect immunoreactivity, so it is important that precise details of the preparation schedule are given when reporting immunocytochemical studies, rather than using the general term "routinely fixed and processed".
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.