ArticleThe EMBO journal1997
Deletions at stalled replication forks occur by two different pathways.
Article in The EMBO journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 76 citations in OpenAlex.
- Genome manipulation by guide-directed Argonaute cleavage.Nucleic acids research · 2023Article
- Mycoplasma Chromosomal Transfer: A Distributive, Conjugative Process Creating an Infinite Variety of Mosaic Genomes.Frontiers in microbiology · 2019Article
- Replication Fork Breakage and Restart in Escherichia coli.Microbiology and molecular biology reviews : MMBR · 2018Review
- Targeting Allostery with Avatars to Design Inhibitors Assessed by Cell Activity: Dissecting MRE11 Endo- and Exonuclease Activities.Methods in enzymology · 2018Article
- Chromosomal Replication Complexity: A Novel DNA Metrics and Genome Instability Factor.PLoS genetics · 2016Review
- Genomic characterization of non-mucus-adherent derivatives of Lactobacillus rhamnosus GG reveals genes affecting pilus biogenesis.Applied and environmental microbiology · 2014Article
- Replication slippage of the thermophilic DNA polymerases B and D from the Euryarchaeota Pyrococcus abyssi.Frontiers in microbiology · 2014Article
- Replication of the Escherichia coli chromosome in RNase HI-deficient cells: multiple initiation regions and fork dynamics.Molecular microbiology · 2014Article
- Mitochondrial DNA deletions are associated with non-B DNA conformations.Nucleic acids research · 2012Article
- Mre11-Rad50-Nbs1 conformations and the control of sensing, signaling, and effector responses at DNA double-strand breaks.DNA repair · 2010Review
- Crystal structure of the first eubacterial Mre11 nuclease reveals novel features that may discriminate substrates during DNA repair.Journal of molecular biology · 2010Article
- Translesion DNA polymerases are required for spontaneous deletion formation in Salmonella typhimurium.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Replication fork barriers: pausing for a break or stalling for time?EMBO reports · 2007Review
- Replication fork stalling at natural impediments.Microbiology and molecular biology reviews : MMBR · 2007Review
- Characterization of in vitro biofilm-associated pneumococcal phase variants of a clinically relevant serotype 3 clone.Journal of clinical microbiology · 2007Article
- Replication termination in Escherichia coli: structure and antihelicase activity of the Tus-Ter complex.Microbiology and molecular biology reviews : MMBR · 2005Review
- Impact of mutS inactivation on foreign DNA acquisition by natural transformation in Pseudomonas stutzeri.Journal of bacteriology · 2005Article
- DNA repair by a Rad22-Mus81-dependent pathway that is independent of Rhp51.Nucleic acids research · 2004Article
- Molecular characteristics of spontaneous deletions in the hyperthermophilic archaeon Sulfolobus acidocaldarius.Journal of bacteriology · 2003Article
- Replication fork collapse at replication terminator sequences.The EMBO journal · 2002Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Replication blockage induces non-homologous deletions in Escherichia coli. The mechanism of the formation of these deletions was investigated. A pBR322-mini-oriC hybrid plasmid carrying two E. coli replication terminators (Ter sites) in opposite orientations was used. Deletions which remove at least the pBR322 blocking site (named Ter1) occurred at a frequency of 2 x 10(-6) per generation. They fall into two equally large classes: deletions that join sequences with no homology, and others that join sequences of 3-10 bp of homology. Some 95% of the deletions in the former class resulted from the fusion of sequences immediately preceding the two Ter sites, indicating a direct role for blocked replication forks in their formation. These deletions were not found in a topA10 mutant, suggesting a topoisomerase I-mediated process. In contrast, deletions joining short homologous sequences were not affected by the topA10 mutation. However, the incidence of this second class of deletions increased 10-fold in a recD mutant, devoid of exonuclease V activity. This indicates that linear molecules are intermediates in their formation. In addition, approximately 50% of these deletions were clustered in the region flanking the Ter1 site. We propose that they are produced by repair of molecules broken at the blocked replication forks.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.