ArticleThe EMBO journal1997
The RLF-M component of the replication licensing system forms complexes containing all six MCM/P1 polypeptides.
Article in The EMBO journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
59 citing papers in PubMed, 138 citations in OpenAlex.
- Origins of DNA replication in eukaryotes.Molecular cell · 2023Review
- Review
- Replication initiation: Implications in genome integrity.DNA repair · 2021Review
- History of DNA Helicases.Genes · 2020Review
- Mechanisms and regulation of DNA replication initiation in eukaryotes.Critical reviews in biochemistry and molecular biology · 2017Review
- Suppression of Reserve MCM Complexes Chemosensitizes to Gemcitabine and 5-Fluorouracil.Molecular cancer research : MCR · 2015Article
- Article
- The Mcm2-7 replicative helicase: a promising chemotherapeutic target.BioMed research international · 2014Review
- Dynamic interaction of Y RNAs with chromatin and initiation proteins during human DNA replication.Journal of cell science · 2011Article
- MCM2-7 form double hexamers at licensed origins in Xenopus egg extract.The Journal of biological chemistry · 2011Article
- Article
- The Mcm complex: unwinding the mechanism of a replicative helicase.Microbiology and molecular biology reviews : MMBR · 2009Review
- Replication licensing and the DNA damage checkpoint.Frontiers in bioscience (Landmark edition) · 2009Review
- The minichromosome maintenance proteins 2-7 (MCM2-7) are necessary for RNA polymerase II (Pol II)-mediated transcription.The Journal of biological chemistry · 2009Article
- Phosphorylation of MCM3 on Ser-112 regulates its incorporation into the MCM2-7 complex.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Minichromosome maintenance proteins 2 and 5 in non-benign epithelial ovarian tumours: relationship with cell cycle regulators and prognostic implications.British journal of cancer · 2007Article
- Phosphorylation of MCM4 at sites inactivating DNA helicase activity of the MCM4-MCM6-MCM7 complex during Epstein-Barr virus productive replication.Journal of virology · 2006Article
- Depletion of minichromosome maintenance protein 5 in the zebrafish retina causes cell-cycle defect and apoptosis.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- The DNA replication factor MCM5 is essential for Stat1-mediated transcriptional activation.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Cdt1 downregulation by proteolysis and geminin inhibition prevents DNA re-replication in Xenopus.The EMBO journal · 2005Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Replication licensing factor (RLF) is involved in preventing re-replication of chromosomal DNA in a single cell cycle, and previously has been separated into two components termed RLF-M and RLF-B. Here we show that Xenopus RLF-M consists of all six members of the MCM/P1 protein family, XMcm2-XMcm7. The six MCM/P1 polypeptides co-eluted on glycerol gradients and gel filtration as complexes with a mol. wt of approximately 400 kDa. In crude Xenopus extract, all six MCM/P1 polypeptides co-precipitated with anti-XMcm3 antibody, although only XMcm5 quantitatively co-precipitated from purified RLF-M. Further fractionation separated RLF-M into two sub-components, one consisting of XMcms 3 and 5, the other consisting of XMcms 2, 4, 6 and 7. Neither of the sub-components provided RLF-M activity. Finally, we show that all six MCM/P1 proteins bind synchronously to chromatin before the onset of S-phase and are displaced as S-phase proceeds. These results strongly suggest that complexes containing all six MCM/P1 proteins are necessary for replication licensing.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.