ArticleThe EMBO journal1997
KARP-1: a novel leucine zipper protein expressed from the Ku86 autoantigen locus is implicated in the control of DNA-dependent protein kinase activity.
Article in The EMBO journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 46 citations in OpenAlex.
- Nuku, a family of primate retrocopies derived from KU70.G3 (Bethesda, Md.) · 2021Article
- The Ku complex: recent advances and emerging roles outside of non-homologous end-joining.Cellular and molecular life sciences : CMLS · 2021Review
- Discovery of DNA repair inhibitors by combinatorial library profiling.Cancer research · 2011Article
- An investigation of hormesis of trichloroethylene in L-02 liver cells by differential proteomic analysis.Molecular biology reports · 2009Article
- The ubiquitin-proteasome system in cancer, a major player in DNA repair. Part 2: transcriptional regulation.Journal of cellular and molecular medicine · 2009Review
- Interleukin-6 affects cell death escaping mechanisms acting on Bax-Ku70-Clusterin interactions in human colon cancer progression.Cell cycle (Georgetown, Tex.) · 2009Article
- The life and death of DNA-PK.Oncogene · 2005Review
- Ku86 autoantigen related protein-1 transcription initiates from a CpG island and is induced by p53 through a nearby p53 response element.Nucleic acids research · 2002Article
- Ku86 is essential in human somatic cells.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Molecular interaction map of the mammalian cell cycle control and DNA repair systems.Molecular biology of the cell · 1999Review
- Ku70/80 gene expression and DNA-dependent protein kinase (DNA-PK) activity do not correlate with double-strand break (dsb) repair capacity and cellular radiosensitivity in normal human fibroblasts.British journal of cancer · 1999Article
- KARP-1 is induced by DNA damage in a p53- and ataxia telangiectasia mutated-dependent fashion.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Interaction of Ku protein and DNA-dependent protein kinase catalytic subunit with nucleic acids.Nucleic acids research · 1998Review
- Cell-cycle regulation of mammalian DNA double-strand-break repair.American journal of human genetics · 1997Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The Ku autoantigen plays an integral role in mammalian DNA double-strand break repair as the DNA binding component of the DNA-dependent protein kinase (DNA-PK) complex. Here, we demonstrate that a second gene, KARP-1 (Ku86 Autoantigen Related Protein-1), is expressed from the Ku86 locus. The KARP-1 gene utilizes an upstream promoter and additional exons which results in an extra 9 kDa of protein appended onto the normal Ku86 polypeptide. The KARP-1-specific domain encodes interdigitating hexa- and penta-heptad repeats of leucine residues flanked by a very basic region. Intriguingly, the catalytic subunit of DNA-PK also contains a hexa-heptad repeat of leucines. Consistent with this observation, we observed that human cell lines stably expressing dominant-negative constructs of KARP-1 resulted in diminished DNA-PK activity and X-ray hypersensitivity and that a KARP-1 antibody significantly neutralized DNA-PK activity in vitro. Finally, we present data which suggests that KARP-1 may be primate-specific. These observations have important repercussions for mammalian DNA double-strand break repair.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.