ArticleThe Journal of cell biology1997
Passive sorting in maturing granules of AtT-20 cells: the entry and exit of salivary amylase and proline-rich protein.
Article in The Journal of cell biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Coding variants identified in patients with diabetes alter PICK1 BAR domain function in insulin granule biogenesis.The Journal of clinical investigation · 2022Article
- A common single nucleotide polymorphism alters the synthesis and secretion of neuropeptide Y.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2008Article
- Not all secretory granules are created equal: Partitioning of soluble content proteins.Molecular biology of the cell · 2006Article
- Parotid secretory granules: crossroads of secretory pathways and protein storage.Journal of dental research · 2005Review
- Is there structural specificity in the reversible protein aggregates that are stored in secretory granules?Journal of molecular neuroscience : MN · 2004Review
- Biogenesis of regulated exocytotic carriers in neuroendocrine cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2000Article
- Targeting of the zymogen-granule protein syncollin in AR42J and AtT-20 cells.The Biochemical journal · 2000Article
- Proinsulin endoproteolysis confers enhanced targeting of processed insulin to the regulated secretory pathway.Molecular biology of the cell · 2000Article
- Efficient binding of regulated secretory protein aggregates to membrane phospholipids at acidic pH.The Biochemical journal · 1999Article
- Sorting of proteins to vacuoles in plant cells.Plant molecular biology · 1998Review
- The protozoan parasite Toxoplasma gondii targets proteins to dense granules and the vacuolar space using both conserved and unusual mechanisms.The Journal of cell biology · 1998Article
- Chromogranin B (secretogranin I), a neuroendocrine-regulated secretory protein, is sorted to exocrine secretory granules in transgenic mice.The EMBO journal · 1998Article
- Sorting and storage during secretory granule biogenesis: looking backward and looking forward.The Biochemical journal · 1998Review
- Enhanced glycosylation and sulfation of secretory proteoglycans is coupled to the expression of a basic secretory protein.Molecular biology of the cell · 1998Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Previous studies have suggested that salivary amylase and proline-rich protein are sorted differently when expressed in AtT-20 cells (Castle, A.M., L.E. Stahl, and J.D. Castle. 1992. J. Biol. Chem. 267:13093- 13100; Colomer, V., K. Lal, T.C. Hoops, and M.J. Rindler. 1994.EMBO (Eur. Mol. Biol. Organ.) J. 13:3711- 3719). We now show that both exocrine proteins behave similarly and enter the regulated secretory pathway as judged by immunolocalization and secretagogue- dependent stimulation of secretion. Analysis of stimulated secretion of newly synthesized proline-rich protein, amylase, and endogenous hormones indicates that the exogenous proteins enter the granule pool with about the same efficiency as the endogenous hormones. However, in contrast to the endogenous hormones, proline-rich protein and amylase are progressively removed from the granule pool during the process of granule maturation such that only small portions remain in mature granules where they colocalize with the stored hormones. The exogenous proteins that are not stored are recovered from the incubation medium and are presumed to have undergone constitutive-like secretion. These results point to a level of sorting for regulated secretion after entry of proteins into forming granules and indicate that retention is essential for efficient storage. Consequently, the critical role of putative sorting receptors for regulated secretion may be in retention rather than in granule entry.
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