ArticleThe Plant cell1997
Epigenetic silencing of a foreign gene in nuclear transformants of Chlamydomonas.
Article in The Plant cell, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 73 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
73 citing papers in PubMed, 204 citations in OpenAlex.
- Integrative framework for selecting gene delivery technologies in microalgae for synthetic biology.Molecular biology reports · 2026Review
- Chromosome-level genome assembly and DNA methylation data of the oleaginous astaxanthin-producing alga Chromochloris zofingiensis.Scientific data · 2026Article
- Organelle-Targeted Multi-Enzyme Engineering Enables Medium-Chain Fatty Acid Production inJournal of microbiology and biotechnology · 2026Article
- Microalgal Synthetic Biotechnology: From Genetic Models to Deployable Photosynthetic Chassis.Research (Washington, D.C.) · 2026Review
- Genome editing in the green alga Chlamydomonas: past, present practice and future prospects.The Plant journal : for cell and molecular biology · 2025Review
- Efficient secretory production of recombinant proteins in microalgae using an exogenous signal peptide.Frontiers in microbiology · 2025Article
- Proteomic analysis reveals molecular changes following genetic engineering in Chlamydomonas reinhardtii.BMC microbiology · 2024Article
- Optimized transgene expression in the red alga Porphyridium purpureum and efficient recombinant protein secretion into the culture medium.Plant molecular biology · 2024Article
- Harnessing genetic engineering to drive economic bioproduct production in algae.Frontiers in bioengineering and biotechnology · 2024Review
- Cell Type-Specific Promoters ofGenes · 2023Article
- Cloning and expression of an anti-cancerous cytokine: human IL-29 gene in Chlamydomonas reinhardtii.AMB Express · 2023Article
- DNA Methylation in Algae and Its Impact on Abiotic Stress Responses.Plants (Basel, Switzerland) · 2023Review
- Site-specific gene knock-in and bacterial phytase gene expression inFrontiers in plant science · 2023Article
- Exploring the Impact of Terminators on Transgene Expression inLife (Basel, Switzerland) · 2021Article
- A novel, robust and mating-competentBiotechnology reports (Amsterdam, Netherlands) · 2021Article
- Tuning SAS-6 architecture with monobodies impairs distinct steps of centriole assembly.Nature communications · 2021Article
- An epigenetic gene silencing pathway selectively acting on transgenic DNA in the green alga Chlamydomonas.Nature communications · 2020Article
- Development of a species-specific transformation system using the novel endogenous promoter calreticulin from oleaginous microalgae Ettlia sp.Scientific reports · 2020Article
- Inhibition of DNA Methylation inFrontiers in genetics · 2020Article
- Good News for Nuclear Transgene Expression inCells · 2019Review
13 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The unstable expression of introduced genes poses a serious problem for the application of transgenic technology in plants. In transformants of the unicellular green alga Chlamydomonas reinhardtii, expression of a eubacterial aadA gene, conferring spectinomycin resistance, is transcriptionally suppressed by a reversible epigenetic mechanism(s). Variations in the size and frequency of colonies surviving on different concentrations of spectinomycin as well as the levels of transcriptional activity of the introduced transgene(s) suggest the existence of intermediate expression states in genetically identical cells. Gene silencing does not correlate with methylation of the integrated DNA and does not involve large alterations in its chromatin structure, as revealed by digestion with restriction endonucleases and DNase I. Transgene repression is enhanced by lower temperatures, similar to position effect variegation in Drosophila. By analogy to epigenetic phenomena in several eukaryotes, our results suggest a possible role for (hetero)chromatic chromosomal domains in transcriptional inactivation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.