ArticleApplied and environmental microbiology1997
Production of succinic acid through overexpression of NAD(+)-dependent malic enzyme in an Escherichia coli mutant.
Article in Applied and environmental microbiology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed, 224 citations in OpenAlex.
- Perspectives for Advancing Biotechnological Succinic Acid Production.Microbial biotechnology · 2026Review
- Metabolic Engineering ofACS synthetic biology · 2025Article
- A synthetic methylotrophicNature catalysis · 2024Article
- Biosynthetic Pathway and Metabolic Engineering of Succinic Acid.Frontiers in bioengineering and biotechnology · 2022Review
- A rotary mechanism for allostery in bacterial hybrid malic enzymes.Nature communications · 2021Article
- Human serum albumin alters specific genes that can play a role in survival and persistence in Acinetobacter baumannii.Scientific reports · 2018Article
- Type and capacity of glucose transport influences succinate yield in two-stage cultivations.Microbial cell factories · 2018Article
- Biological production of L-malate: recent advances and future prospects.World journal of microbiology & biotechnology · 2017Review
- Holistic bioengineering: rewiring central metabolism for enhanced bioproduction.The Biochemical journal · 2017Review
- Robust mutant strain design by pessimistic optimization.BMC genomics · 2017Article
- Redirecting carbon flux through pgi-deficient and heterologous transhydrogenase toward efficient succinate production in Corynebacterium glutamicum.Journal of industrial microbiology & biotechnology · 2017Article
- Efficient estimation of the maximum metabolic productivity of batch systems.Biotechnology for biofuels · 2017Article
- High-yield anaerobic succinate production by strategically regulating multiple metabolic pathways based on stoichiometric maximum in Escherichia coli.Microbial cell factories · 2016Article
- Targeted optimization of central carbon metabolism for engineering succinate production in Escherichia coli.BMC biotechnology · 2016Article
- C4-Dicarboxylate Utilization in Aerobic and Anaerobic Growth.EcoSal Plus · 2016Article
- In silico profiling of cell growth and succinate production inBioresources and bioprocessing · 2016Article
- Production of the Bioactive Compounds Violacein and Indolmycin Is Conditional in aFrontiers in microbiology · 2016Article
- Metabolic engineering of carbon and redox flow in the production of small organic acids.Journal of industrial microbiology & biotechnology · 2015Review
- NADPH-generating systems in bacteria and archaea.Frontiers in microbiology · 2015Review
- Engineering microorganisms based on molecular evolutionary analysis: a succinate production case study.Evolutionary applications · 2014Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
NAD(+)-dependent malic enzyme was cloned from the Escherichia coli genome by PCR based on the published partial sequence of the gene. The enzyme was overexpressed and purified to near homogeneity in two chromatographic steps and was analyzed kinetically in the forward and reverse directions. The Km values determined in the presence of saturating cofactor and manganese ion were 0.26 mM for malate (physiological direction) and 16 mM for pyruvate (reverse direction). When malic enzyme was induced under appropriate culture conditions in a strain of E. coli that was unable to ferment glucose and accumulated pyruvate, fermentative metabolism of glucose was restored. Succinic acid was the major fermentation product formed. When this fermentation was performed in the presence of hydrogen, the yield of succinic acid increased. The constructed pathway represents an alternative metabolic route for the fermentative production of dicarboxylic acids from renewable feedstocks.
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