ArticleApplied and environmental microbiology1997
Cloning and characterization of two rhamnogalacturonan hydrolase genes from Aspergillus niger.
Article in Applied and environmental microbiology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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8 citing papers in PubMed, 37 citations in OpenAlex.
- Expression-based clustering of CAZyme-encoding genes of Aspergillus niger.BMC genomics · 2017Article
- Mapping the polysaccharide degradation potential of Aspergillus niger.BMC genomics · 2012Article
- Fungal enzyme sets for plant polysaccharide degradation.Applied microbiology and biotechnology · 2011Review
- Leaf-cutting ant fungi produce cell wall degrading pectinase complexes reminiscent of phytopathogenic fungi.BMC biology · 2010Article
- Enzymatic deconstruction of backbone structures of the ramified regions in pectins.The protein journal · 2008Review
- A new group of exo-acting family 28 glycoside hydrolases of Aspergillus niger that are involved in pectin degradation.The Biochemical journal · 2006Article
- Aspergillus enzymes involved in degradation of plant cell wall polysaccharides.Microbiology and molecular biology reviews : MMBR · 2001Review
- Endo-xylogalacturonan hydrolase, a novel pectinolytic enzyme.Applied and environmental microbiology · 2000Article
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6 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
A rhamnogalacturonan hydrolase gene of Aspergillus aculeatus was used as a probe for the cloning of two rhamnogalacturonan hydrolase genes of Aspergillus niger. The corresponding proteins, rhamnogalacturonan hydrolases A and B, are 78 and 72% identical, respectively, with the A. aculeatus enzyme. In A. niger cultures which were shifted from growth on sucrose to growth on apple pectin as a carbon source, the expression of the rhamnogalacturonan hydrolase A gene (rhgA) was transiently induced after 3 h of growth on apple pectin. The rhamnogalacturonan hydrolase B gene was not induced by apple pectin, but the rhgB gene was derepressed after 18 h of growth on either apple pectin or sucrose. Gene fusions of the A. niger rhgA and rhgB coding regions with the strong and inducible Aspergillus awamori exlA promoter were used to obtain high-producing A. awamori transformants which were then used for the purification of the two A. niger rhamnogalacturonan hydrolases. High-performance anion-exchange chromatography of oligomeric degradation products showed that optimal degradation of an isolated highly branched pectin fraction by A. niger rhamnogalacturonan hydrolases A and B occurred at pH 3.6 and 4.1, respectively. The specific activities of rhamnogalacturonan hydrolases A and B were then 0.9 and 0.4 U/mg, respectively, which is significantly lower than the specific activity of A. aculeatus rhamnogalacturonan hydrolase (2.5 U/mg at an optimal pH of 4.5). Compared to the A enzymes, the A. niger B enzyme appears to have a different substrate specificity, since additional oligomers are formed.
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