ArticleThe Biochemical journal1997
Increased flux through the hexosamine biosynthesis pathway inhibits glucose transport acutely by activation of protein kinase C.
Article in The Biochemical journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 64 citations in OpenAlex.
- A Review on Oxidative Stress, Diabetic Complications, and the Roles of Honey Polyphenols.Oxidative medicine and cellular longevity · 2020Review
- Hyperglycemia-induced downregulation of apolipoprotein M expression is not via the hexosamine pathway.Lipids in health and disease · 2015Article
- Glutamine and glutamate limit the shortening of action potential duration in anoxia-challenged rabbit hearts.Physiological reports · 2015Article
- Differential metabolic effects of glucosamine and N-acetylglucosamine in human articular chondrocytes.Osteoarthritis and cartilage · 2009Article
- 2,5-Deoxyfructosazine, a D-glucosamine derivative, inhibits T-cell interleukin-2 production better than D-glucosamine.Carbohydrate research · 2007Article
- Glucosamine-induced increase in Akt phosphorylation corresponds to increased endoplasmic reticulum stress in astroglial cells.Molecular and cellular biochemistry · 2007Article
- The hexosamine biosynthesis pathway negatively regulates IL-2 production by Jurkat T cells.Cellular immunology · 2007Article
- Diabetic cardiomyocyte dysfunction and myocyte insulin resistance: role of glucose-induced PKC activity.Molecular and cellular biochemistry · 2004Article
- Peripheral insulin resistance develops in transgenic rats overexpressing phosphoenolpyruvate carboxykinase in the kidney.Diabetologia · 2003Article
- P38 mitogen-activated protein kinase mediates hexosamine-induced TGFbeta1 mRNA expression in human mesangial cells.Diabetologia · 2003Article
- Article
- Autocrine and paracrine mechanisms in the early stages of diabetic nephropathy.Journal of endocrinological investigation · 1999Review
- Possible role for gp160 in constitutive but not insulin-stimulated GLUT4 trafficking: dissociation of gp160 and GLUT4 localization.The Biochemical journal · 1998Article
Corrections and comments
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Authors and funding
3 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The hexosamine biosynthesis pathway and protein kinase C (PKC) activation mediate hyperglycaemia-induced impaired glucose transport, but the relative role of each pathway is unknown. Following a 2 h preincubation of rat adipocytes in the presence of either high glucose (30 mM) plus insulin (0.7 nM) or glucosamine (3 mM), both high glucose and glucosamine inhibited subsequent basal and insulin-stimulated glucose transport, measured at 5.0 mM glucose. Azaserine, an inhibitor of the enzyme glutamine:fructose-6-phosphate aminotransferase, abolished the effect of high glucose, but not that of glucosamine. Ro-31-8220, an inhibitor of PKC, reversed the effects of both high glucose and glucosamine, suggesting that flux through the hexosamine biosynthesis pathway impaired glucose transport acutely by activating PKC. Both high glucose and glucosamine caused a 3-fold increase in PKC activity; this effect of high glucose, but not that of glucosamine, was partially decreased by azaserine. Neither high glucose nor glucosamine altered basal or insulin-stimulated plasma membrane GLUT1 levels, whereas both treatments decreased basal, but not insulin-stimulated, GLUT4 levels. Azaserine abolished the effect of high glucose, but not that of glucosamine, on basal plasma membrane GLUT4 levels. Ro-31-8220, which returned glucose transport to control values, caused a further decrease in plasma membrane GLUT4 levels. It is concluded that, in rat adipocytes, an acute increase in flux through the hexosamine biosynthesis pathway inhibits glucose transport by activation of PKC.
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