ArticleJournal of bacteriology1997
Promoter selectivity of Escherichia coli RNA polymerase sigmaF holoenzyme involved in transcription of flagellar and chemotaxis genes.
Article in Journal of bacteriology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 59 citations in OpenAlex.
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- Mechanism ofInternational journal of molecular sciences · 2023Article
- Cross-Recognition of Promoters by the Nine SigB Homologues Present inInternational journal of molecular sciences · 2021Article
- Expanded roles of pyruvate-sensing PdhR in transcription regulation of theMicrobial genomics · 2020Article
- Structural basis of bacterial σThe EMBO journal · 2020Article
- Interactions between DksA and Stress-Responsive Alternative Sigma Factors Control Inorganic Polyphosphate Accumulation in Escherichia coli.Journal of bacteriology · 2020Article
- Modelling and measuring intracellular competition for finite resources during gene expression.Journal of the Royal Society, Interface · 2019Article
- Essential Role of σ Factor RpoF in Flagellar Biosynthesis and Flagella-Mediated Motility ofFrontiers in microbiology · 2019Article
- Membrane Curvature and the Tol-Pal Complex Determine Polar Localization of the Chemoreceptor Tar in Escherichia coli.Journal of bacteriology · 2018Article
- The whole set of the constitutive promoters recognized by four minor sigma subunits of Escherichia coli RNA polymerase.PloS one · 2017Article
- The transcriptional repressor EUO regulates both subsets of Chlamydia late genes.Molecular microbiology · 2014Article
- Engineering bacterial transcription regulation to create a synthetic in vitro two-hybrid system for protein interaction assays.Journal of the American Chemical Society · 2014Article
- A model for sigma factor competition in bacterial cells.PLoS computational biology · 2014Article
- Comprehensive mapping of the Escherichia coli flagellar regulatory network.PLoS genetics · 2014Article
- Mycobacterium tuberculosis RbpA protein is a new type of transcriptional activator that stabilizes the σ A-containing RNA polymerase holoenzyme.Nucleic acids research · 2012Article
- Isolation and characterization of Ehrlichia chaffeensis RNA polymerase and its use in evaluating p28 outer membrane protein gene promoters.BMC microbiology · 2011Article
- In vitro genetic reconstruction of bacterial transcription initiation by coupled synthesis and detection of RNA polymerase holoenzyme.Nucleic acids research · 2010Article
- TraR, a homolog of a RNAP secondary channel interactor, modulates transcription.PLoS genetics · 2009Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The rpoF gene of Escherichia coli codes for the RNA polymerase sigmaF (or sigma28) subunit, which is involved in transcription of the flagellar and chemotaxis genes. Both sigmaF and sigma70 (the major sigma subunit in growing cells) were overexpressed, purified to homogeneity, and compared with respect to activity and specificity. The affinity of sigmaF to core RNA polymerase (E) is higher than that of sigma70, as measured by gel filtration high-pressure liquid chromatography. In an in vitro transcription system, the holoenzyme (E sigmaF) containing sigmaF selectively transcribed the flagellar and chemotaxis genes, all of which could not be transcribed by E sigma70. This strict promoter recognition property of sigmaF is similar to those of other stress response minor sigma subunits but different from those of the principal sigma subunits, sigma70 and sigma38. sigma70-dependent transcription in vitro is inhibited at high concentrations of all salts tested, showing maximum activity at 50 mM. In contrast, sigmaF-dependent transcription was maximum at 50 mM KCI and then decreased to negligible level at 300 mM; in the cases of potassium acetate and potassium glutamate, maximum transcription was between 200 and 300 mM. DNase I foot printing of the fliC and fliD promoters indicated that sigmaF alone is unable to bind DNA, but E sigmaF specifically recognizes -10 and -35 regions of the sigmaF-dependent promoters with rather long upstream protection. Alteration of the promoter structure after binding of E sigmaF was suggested.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.