ArticleJournal of bacteriology1997
Duplication of the pepF gene and shuffling of DNA fragments on the lactose plasmid of Lactococcus lactis.
Article in Journal of bacteriology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 43 citations in OpenAlex.
- A Novel Regulator PepR Regulates the Expression of Dipeptidase GeneMicroorganisms · 2024Article
- Phenotypic, Technological, Safety, and Genomic Profiles of Gamma-Aminobutyric Acid-ProducingInternational journal of molecular sciences · 2024Article
- Competence shut-off by intracellular pheromone degradation in salivarius streptococci.PLoS genetics · 2022Article
- Role of bacterial peptidase F inferred by statistical analysis and further experimental validation.HFSP journal · 2008Article
- An unstable competence-induced protein, CoiA, promotes processing of donor DNA after uptake during genetic transformation in Streptococcus pneumoniae.Journal of bacteriology · 2006Article
- Enzymatic ability of Bifidobacterium animalis subsp. lactis to hydrolyze milk proteins: identification and characterization of endopeptidase O.Applied and environmental microbiology · 2005Article
- Complete sequences of four plasmids of Lactococcus lactis subsp. cremoris SK11 reveal extensive adaptation to the dairy environment.Applied and environmental microbiology · 2005Article
- Two thimet oligopeptidase-like Pz peptidases produced by a collagen-degrading thermophile, Geobacillus collagenovorans MO-1.Journal of bacteriology · 2005Article
- Identification of novel restriction endonuclease-like fold families among hypothetical proteins.Nucleic acids research · 2005Article
- Identification and characterization of Lactobacillus helveticus PepO2, an endopeptidase with post-proline specificity.Applied and environmental microbiology · 2003Article
- The peptidyl-prolyl isomerase motif is lacking in PmpA, the PrsA-like protein involved in the secretion machinery of Lactococcus lactis.Applied and environmental microbiology · 2002Article
- Overexpression of the PepF oligopeptidase inhibits sporulation initiation in Bacillus subtilis.Journal of bacteriology · 2002Article
- Transcriptional pattern of genes coding for the proteolytic system of Lactococcus lactis and evidence for coordinated regulation of key enzymes by peptide supply.Journal of bacteriology · 2001Article
- Genome plasticity among related ++Lactococcus strains: identification of genetic events associated with macrorestriction polymorphisms.Journal of bacteriology · 2000Article
- Genetic characterization and physiological role of endopeptidase O from Lactobacillus helveticus CNRZ32.Applied and environmental microbiology · 1998Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The gene corresponding to the lactococcal oligopeptidase PepF1 (formerly PepF [V. Monnet, M. Nardi, A. Chopin, M.-C. Chopin, and J.-C. Gripon, J. Biol. Chem. 269:32070-32076, 1994]) is located on the lactose-proteinase plasmid of Lactococcus lactis subsp. cremoris NCDO763. Use of the pepF1 gene as a probe with different strains showed that pepF1 is present on the chromosome of Lactococcus lactis subsp. lactis IL1403, whereas there is a second, homologous gene, pepF2, on the chromosome of strain NCDO763. From hybridization, PCR amplification, and sequencing experiments, we deduced that (i) pepF1 and pepF2 exhibit 80% identity and encode two proteins which are 84% identical and (ii) pepF2 is included in an operon composed of three open reading frames and is transcribed from two promoters. The protein, encoded by the gene located downstream of pepF2, shows significant homology with methyltransferases. Analysis of the sequences flanking pepF1 and pepF2 indicates that only a part of the pepF2 operon is present on the plasmid of strain NCDO763, while the operon is intact on the chromosome of strain IL1403. Traces of several recombination events are visible on the lactose-proteinase plasmid. This suggests that the duplication of pepF occurred by recombination from the chromosome of an L. lactis subsp. lactis strain followed by gene transfer. We discuss the possible functions of PepF and the role of its amplification.
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