ArticleProceedings of the National Academy of Sciences of the United States of America1997
Gene replacement by homologous recombination in the multicellular green alga Volvox carteri.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 59 citations in OpenAlex.
- Cell Type-Specific Promoters ofGenes · 2023Article
- Transgene Expression in Microalgae-From Tools to Applications.Frontiers in plant science · 2016Review
- The inducible nitA promoter provides a powerful molecular switch for transgene expression in Volvox carteri.BMC biotechnology · 2015Article
- Stable nuclear transformation of Pandorina morum.BMC biotechnology · 2014Article
- Stable nuclear transformation of Eudorina elegans.BMC biotechnology · 2013Article
- Evolution of reproductive development in the volvocine algae.Sexual plant reproduction · 2011Review
- Stable nuclear transformation of Gonium pectorale.BMC biotechnology · 2009Article
- Idaten is a new cold-inducible transposon of Volvox carteri that can be used for tagging developmentally important genes.Genetics · 2008Article
- Transformation of the green alga Haematococcus pluvialis with a phytoene desaturase for accelerated astaxanthin biosynthesis.Applied and environmental microbiology · 2006Article
- Swapped green algal promoters: aphVIII-based gene constructs with Chlamydomonas flanking sequences work as dominant selectable markers in Volvox and vice versa.Plant cell reports · 2006Article
- Chimeric RNA/DNA oligonucleotide-based site-specific modification of the tobacco acetolactate syntase gene.Plant physiology · 2003Article
- A link between DNA methylation and epigenetic silencing in transgenic Volvox carteri.Nucleic acids research · 2001Article
- Volvoxrhodopsin, a light-regulated sensory photoreceptor of the spheroidal green alga Volvox carteri.The Plant cell · 1999Article
- Plant nuclear gene knockout reveals a role in plastid division for the homolog of the bacterial cell division protein FtsZ, an ancestral tubulin.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
With only two different cell types, the haploid green alga Volvox represents the simplest multicellular model system. To facilitate genetic investigations in this organism, the occurrence of homologous recombination events was investigated with the intent of developing methods for gene replacement and gene disruption. First, homologous recombination between two plasmids was demonstrated by using overlapping nonfunctional fragments of a recombinant arylsulfatase gene (tubulin promoter/arylsulfatase gene). After bombardment of Volvox reproductive cells with DNA-coated gold microprojectiles, transformants expressing arylsulfatase constitutively were recovered, indicating the presence of the machinery for homologous recombination in Volvox. Second, a well characterized loss-of-function mutation in the nuclear nitrate reductase gene (nitA) with a single G --> A nucleotide exchange in a 5'-splice site was chosen as a target for gene replacement. Gene replacement by homologous recombination was observed with a reasonably high frequency only if the replacement vector containing parts of the functional nitrate reductase gene contained only a few nucleotide exchanges. The ratio of homologous to random integration events ranged between 1:10 and 1:50, i.e., homologous recombination occurs frequently enough in Volvox to apply the powerful tool of gene disruption for functional studies of novel genes.
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