ArticleProceedings of the National Academy of Sciences of the United States of America1997
Distinct mechanisms of splicing regulation in vivo by the Drosophila protein Sex-lethal.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 35 citations in OpenAlex.
- Biochemical and functional analysis of Drosophila-sciara chimeric sex-lethal proteins.PloS one · 2013Article
- The translation initiation factor eIF4E regulates the sex-specific expression of the master switch gene Sxl in Drosophila melanogaster.PLoS genetics · 2011Article
- A conditional role of U2AF in splicing of introns with unconventional polypyrimidine tracts.Molecular and cellular biology · 2007Article
- The gene Sex-lethal of the Sciaridae family (order Diptera, suborder Nematocera) and its phylogeny in dipteran insects.Genetics · 2004Article
- The conserved RNA recognition motif 3 of U2 snRNA auxiliary factor (U2AF 65) is essential in vivo but dispensable for activity in vitro.RNA (New York, N.Y.) · 2004Article
- RNA binding protein sex-lethal (Sxl) and control of Drosophila sex determination and dosage compensation.Microbiology and molecular biology reviews : MMBR · 2003Review
- U2 small nuclear ribonucleoprotein particle (snRNP) auxiliary factor of 65 kDa, U2AF65, can promote U1 snRNP recruitment to 5' splice sites.The Biochemical journal · 2003Article
- Sex lethal and U2 small nuclear ribonucleoprotein auxiliary factor (U2AF65) recognize polypyrimidine tracts using multiple modes of binding.RNA (New York, N.Y.) · 2003Article
- SRp30c is a repressor of 3' splice site utilization.Molecular and cellular biology · 2002Article
- RNA-protein interactions that regulate pre-mRNA splicing.Gene expression · 2002Review
- Modulation of msl-2 5' splice site recognition by Sex-lethal.RNA (New York, N.Y.) · 2001Article
- Polypyrimidine tract binding protein antagonizes exon definition.Molecular and cellular biology · 2001Review
- Polypyrimidine track-binding protein binding downstream of caspase-2 alternative exon 9 represses its inclusion.The Journal of biological chemistry · 2001Article
- Switch in 3' splice site recognition between exon definition and splicing catalysis is important for sex-lethal autoregulation.Molecular and cellular biology · 2001Article
- Differential recognition of the polypyrimidine-tract by the general splicing factor U2AF65 and the splicing repressor sex-lethal.RNA (New York, N.Y.) · 2000Article
- Article
- Absence of interdomain contacts in the crystal structure of the RNA recognition motifs of Sex-lethal.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- An N-terminal truncation uncouples the sex-transforming and dosage compensation functions of sex-lethal.Molecular and cellular biology · 1999Article
- Article
- The Drosophila splicing regulator sex-lethal directly inhibits translation of male-specific-lethal 2 mRNA.RNA (New York, N.Y.) · 1998Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The protein Sex-lethal (SXL) controls pre-mRNA splicing of two genes involved in Drosophila sex determination: transformer (tra) and the Sxl gene itself. Previous in vitro results indicated that SXL antagonizes the general splicing factor U2AF65 to regulate splicing of tra. In this report, we have used transgenic flies expressing chimeric proteins between SXL and the effector domain of U2AF65 to study the mechanisms of splicing regulation by SXL in vivo. Conferring U2AF activity to SXL relieves its inhibitory activity on tra splicing but not on Sxl splicing. Therefore, antagonizing U2AF65 can explain tra splicing regulation both in vitro and in vivo, but this mechanism cannot explain splicing regulation of Sxl pre-mRNA. These results are a direct proof that Sxl, the master regulatory gene in sex determination, has multiple and separable activities in the regulation of pre-mRNA splicing.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.