Evidence map›Paper›PMID 9207047›Full record

ArticleNucleic acids research1997

Efficient recovery and regeneration of integrated retroviruses.

B Schott, E S Kandel, I B Roninson

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nucleic acids research, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 46% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 9 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

B SchottDepartment of Genetics (M/C 669), University of Illinois at Chicago, 900 South Ashland Avenue, Chicago, IL 60607-7170, USA.
E S Kandel
I B Roninson
University of Illinois Chicago · US

Funding

TARGETING TUMOR CELLS WITH GENETIC SUPPRESSOR ELEMENTSR01CA062099 · NCI · UNIVERSITY OF ILLINOIS AT CHICAGO · PI RONINSON, IGOR B · 1994 to 2000
$215k
RECESSIVE MECHANISIMS OF RESISTANCE TO ANTICANCER DRUGSR01CA056736 · NCI · UNIVERSITY OF ILLINOIS AT CHICAGO · PI RONINSON, IGOR B · 1992 to 1996
–
NCI NIH HHS R01-CA56736NCI NIH HHS R01-CA62099
6 · The paper itself

Abstract

We report a rapid and efficient PCR-based rescue procedure for integrated recombinant retroviruses. Full-length proviral DNA is amplified by long-range PCR using a pair of primers derived from the long terminal repeats (LTR), and virus is regenerated by transfecting retrovirus-packaging cells with the PCR-derived provirus. The viral yield from the PCR product is similar to that from the retroviral plasmid vector, and the representation of different inserts is accurately maintained in the recovered retroviral population. This procedure is suitable for expression cloning from retroviral libraries and should be applicable to the analysis of natural retrovirus populations.

Indexed as

Genetic VectorsVirus IntegrationCloning, MolecularHumansMoloney murine leukemia virusPolymerase Chain ReactionSarcoma Viruses, MurineTumor Cells, Cultured

Identifiers

PMID9207047
PMCPMC146804
OpenAlexW2144519272

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.