ArticleNucleic acids research1997
Overexpression in COS cells of p50, the major core protein associated with mRNA, results in translation inhibition.
Article in Nucleic acids research, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 101 citations in OpenAlex.
- Y-Box Binding Proteins in mRNP Assembly, Translation, and Stability Control.Biomolecules · 2020Review
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- shRNA-Mediated Silencing of Y-Box Binding Protein-1 (YB-1) Suppresses Growth of Neuroblastoma Cell SH-SY5Y In Vitro and In Vivo.PloS one · 2015Article
- A murine retrovirus co-Opts YB-1, a translational regulator and stress granule-associated protein, to facilitate virus assembly.Journal of virology · 2014Article
- The proteolytic YB-1 fragment interacts with DNA repair machinery and enhances survival during DNA damaging stress.Cell cycle (Georgetown, Tex.) · 2013Article
- YB-1 stabilizes HIV-1 genomic RNA and enhances viral production.Protein & cell · 2013Article
- An intercellular polyamine transfer via gap junctions regulates proliferation and response to stress in epithelial cells.Molecular biology of the cell · 2013Article
- Identification of mRNAs bound and regulated by human LIN28 proteins and molecular requirements for RNA recognition.RNA (New York, N.Y.) · 2013Article
- Control of translation and miRNA-dependent repression by a novel poly(A) binding protein, hnRNP-Q.PLoS biology · 2013Article
- Macromolecular crowding regulates assembly of mRNA stress granules after osmotic stress: new role for compatible osmolytes.The Journal of biological chemistry · 2012Article
- An integrative approach to identify YB-1-interacting proteins required for cisplatin resistance in MCF7 and MDA-MB-231 breast cancer cells.Cancer science · 2011Article
- Role of microtubules in stress granule assembly: microtubule dynamical instability favors the formation of micrometric stress granules in cells.The Journal of biological chemistry · 2009Article
- General RNA-binding proteins have a function in poly(A)-binding protein-dependent translation.The EMBO journal · 2009Article
- YB-1 promotes microtubule assembly in vitro through interaction with tubulin and microtubules.BMC biochemistry · 2008Article
- Requirement of the protein B23 for nucleolar disassembly induced by the FRGY2a family proteins.The Journal of biological chemistry · 2006Article
- Akt-mediated YB-1 phosphorylation activates translation of silent mRNA species.Molecular and cellular biology · 2006Article
- Regulation of RNA splicing by the methylation-dependent transcriptional repressor methyl-CpG binding protein 2.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Proteasome-mediated cleavage of the Y-box-binding protein 1 is linked to DNA-damage stress response.The EMBO journal · 2005Article
- mRNA with a <20-nt poly(A) tail imparted by the poly(A)-limiting element is translated as efficiently in vivo as long poly(A) mRNA.RNA (New York, N.Y.) · 2005Article
- Absence of the DNA-/RNA-binding protein MSY2 results in male and female infertility.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 2 countries.
Funding
Abstract
p50, the major core protein of messenger ribonucleoprotein particles (mRNPs) in the cytoplasm of somatic mammalian cells, has been characterized previously as a member of the Y-box binding transcription factor family of proteins (YB-protein) by both high structural homology and ability to bind specifically the Y-box sequence in double-stranded DNA. YB proteins are present in a whole range of cell types and some have been identified as germ-specific cytoplasmic proteins masking stored mRNA from translation. Western blot analysis of the distribution of p50 in subcellular fractions of COS-1 cells shows that p50 is a cytoplasmic protein quantitatively associated with mRNA, both in polyribosomes and in free mRNPs. The level of p50 in COS-1 cells determined by Western immunoblotting is 0.10% of total protein, which is nearly equimolar to that of ribosomes and is approximately 5-10-fold higher than the mRNA level. Transient transfection of COS-1 cells with a p50-expressing vector results in a dramatic inhibition of protein synthesis. A control transfection with a vector expressing a frameshift mutant of p50 does not cause translation inhibition. Therefore the increase in p50 protein level is responsible for the inhibitory effect in these cells.
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